• Home
  • The effect of –hydroxybutyrate and estradiol on the process of phagocytosis of ovine neutrophils in vitro

Share To

Article Url


Manuscript ID : 518366 Visit : 331 Page: 209 - 215

Article Type: Original Research

The effect of –hydroxybutyrate and estradiol on the process of phagocytosis of ovine neutrophils in vitro

Subject Areas : Veterinary Clinical Pathology

Ali Rezapour 1 , جعفر Majidi 2 , مرتضی Tahmouzy 3

1 - Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Tabriz Branch, Tabriz, Iran.
2 - Research Center of Infectious Disease, Tabriz University of Medical Science, Tabriz, Iran.
3 - Department of Animal Science, Faculty of Agriculture, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

Received: 2008-10-30 Accepted : 2009-01-19 Published : 2008-11-21

Keywords: estradiol, phagocytosis, Nitroblue tetrazolium reduction test, beta-hydroxybutyrate,

Abstract :

     The object of this study was to evaluate the independent effect different concentration of beta-hydroxybutyrate (2.4 & 4.8 mmol/ml) and estradiol (20&400 pg/ml) on the functions phagocytosis and Nitroblue tetrazolium reduction test or respiratory burst of isolated ovine neutrophils in vitro. Isolated neutrophils of sheep were incubated for one hour in room temperature with the previously treatments. The study consisted of 7 treatments in 5 replicates and both phagocytosis and NBT tests of tests were performed in every treatment. The Results of this study indicated that 2.4 & 4.8 mmol/ml concentrations of beta-hydroxybutyrate and estrodiol concentrations of 20 & 400 pg/ml significantly (p<0.01) reduced the percentage of phagocytosis and NBT reduction test  of sheep neutrophils and this could be the reason for the suppression of the immune system in peripartureint  sheep.

References:


  1. رضاپور، ع.، صافی، ش.، مجیدی، ج. و سهرابی حقدوست، ا. (1386): تأثیر بتاهیدروکسی بوتیرات و استروژن بر فرآیند فاگوسیتوز نوتروفیل‌های گاو به‌صورت in-vitro، مجله علوم دامپزشکی ایران، سال چهار، دوره 2، صفحات: 102- 95.
    2.
  2. Arthur, G.H., Noakes, D.E., Pearson, H. and Parkinson, T.J. (1996): Veterinary Reproduction & Obstetrics. Saunders, London, pp: 73-98.
    3.
  3. Booth, N.H. and McDonald, L.E. (1988): Veterinary Pharmacology and Therapeutics. 6th ed., Iowa State University Press, USA, pp: 598-607.
    4.
  4. Brousseau, P., Payette, Y. and Tryphonas, H. (1999): Manual of Immunological Methods. Taylor & Francis CRC Press, pp: 20-45.
    5.
  5. Chanarian, I. (1989): Laboratory Hematology (an account of laboratory techniques). Churchill Livingstone, pp: 9-12 & 177-178.
    6.
  6. Durán, M.G., Gálvez, G.G., de Frutos, T., Recaséns, J.D., Casado, S. and Farré, A.P. (2000): 17 Beta-estradiol-stimulated nitric oxide productions by neutrophils: effect on platelet activation. Obstetrics and gynecology, 95: 284-290.
    7.
  7. Feldman, B.F., Zinkl, J.G. and Jain, N.C. (2000): Schalm’s Veterinary Hematology. 5th ed., Lippincott Williams & Wilkins, Chapters: 46, 52, 54 & 57.
    8.
  8. Gooi, H.G. and Chapel, H. (1990): Clinical Immunology (a practical approach). Oxford University Press, pp: 51-80.
    9.
  9. Grohn, Y., Lidberg, L.A., Bruss, M.L. and Farver, T.B. (1983): Fatty infiltration of liver in spontaneously ketotic dairy cows. J. Dairy Sci., 66: 2320–2328.
    10.
  10. Kaneko, J.J., Harvey, J.W. and Bruss, M.L. (1997): Clinical Biochemistry of Domestic Animals. 5th ed., Academic Press, San Diego, Chapter 3.
    11.
  11. Kauppinen, K. (1983): Prevalence of bovine ketosis in relation to number and stage of lactation. Acta Vet. Scand., 24: 349–361.
    12.
  12. Hafez, E.S.E. and Hafez, B. (2000): Immunology of Reproduction In: Reproduction in Farm Animals. 7th ed., Lippincott Williams & Wilkins, pp: 341-353.
    13.
  13. McMurray, R.W. (2001): Estrogen, prolactin, and autoimmunity: actions and interactions (a review). International Immunopharmacology, 1: 995-1008.
    14.
  14. Molloy, E.J., O’neill, A.J., Grantham, J.J., Sheridan-Pereira, M., Fitzpatrick, J.M., Webb, D.W. and Watson, R.W.G. (2003): Sex-specific alterations in neutrophil apoptosis: the role of estradiol and progesterone. Blood, 102 (7): 2653-2659.
    15.
  15. Monfardini, E., Paape, M.J., Wang, Y., Capuco, A.V., Husheem, M., Wood, L. and Burvenich, C. (2002): Evaluation of L-selectin expression and assessment of protein tyrosine phosphorylation in bovine polymorphonuclear neutrophil leukocytes around parturition. Veterinary Research, 33: 271-281.
    16.
  16. Roth, J.A., Kaeberle, M.L. and Hsu, W.H. (1982): Effect of estradiol and progesterone on lymphocyte and neutrophil functions in steers. Infection and Immunity, pp: 997-1002.
    17.
  17. Roth, J.A. and Nachreiner, R.F. (1983): Association of increased estradiol and progesterone blood values with altered bovine polymorphonuclear leukocyte function. American Journal of Veterinary Medicine, 44 (2): 247-253.
    18.
  18. Sartorelli, P., Paltrinieri, S. and Agnes, F. (1999): Non-specific immunity and ketone bodies. I: In vitro studies on chemotaxis and phagocytosis in ovine neutrophils. Am. J. Vet. Med., A, 46: 613–619.
    19.
  19. Sartorelli, P., Paltrinieri, S. and Comazzi, S. (2000): Non-specific immunity and ketone bodies. II: In vitro studies on adherence and superoxide anion production in ovine neutrophils. Am. J. Vet. Med., A 47: 1–8.
    20.
  20. Sato, N., Shimizu, H., Shimomura, Y., Suwa, K., Mori, M. and Kobayashi, I. (1992): Mechanism of inhibitory action of ketone bodies on the production of reactive oxygen intermediates (ROIS) by polymorphonuclear leukocytes. Life Sci., 51: 113–118.
    21.
  21. Stockham, S.L. and Scott, M.A. (2004): Fundamentals of Veterinary Clinical Pathology. Iowa State University Press, USA, pp: 50-80.
    22.
  22. Strzemienski, P.J., Dyer, R.M. and Kenny, R.M. (1987): Effect of estradiol and progesterone on anti-staphylococal activity of neutrophils from ovariectomized mares. Am. J. Vet. Res., 48: 1638-1641.
    23.
  23. Suriayasathaporn, W., Heuer, C., Noordhuizen-Stassen, E.N. and Schukken, Y.H. (2000): Hyperketonemia and the impairment of udder defense: a review. Vet. Res., 31: 397-412.
    24.
  24. Van Merris, V., Meyer, E., Duchateau, L. and Burvenich, C. (2004): Differential effects of steroids and retinoids on bovine myelopoiesis in vitro. Journal of Dairy Science, 87: 1188–1195
    25.
  25. Weber, P.S., Toelboell, T., Chang, L.C., Tirrell, J.D., Saama, P.M., Smith, G.W. and Burton, J.L. (2004): Mechanism of glucocorticoid-induced down-regulation of neutrophil L-selectin in cattle: evidence for effects at the gene-expression level and primarily on blood neutrophils. Journal of leukocyte biology, 75: 815-827.
    26.
  26. Winters,K.R.H., Meyer, E., Van Merris, V.M., Van Den Broeck, W.L.M., Duchateau, L. and Burvenich, C. (2003): Sex steroid hormones do not influence the oxidative burst activity of polymorphonuclear leukocytes from ovariectomized cows in vitro. Steroids, 68: 397–406.
    27.

 

 

Related articles

The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024

Home| Login| About Us| Contact Us|
[فارسی] [العربية] [fa] [ar]