Effect of supplementing ascorbic acid, vitamin E and betaine on the performance, immune response and blood parameters in broiler
Subject Areas :
Veterinary Clinical Pathology
S.M
Motamedi
1
(Ph.D. Student of Animal and Poultry Nutrition, Department of Animal Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Lecturer of Payam-e-Noor University, Tehran, Iran.)
Received: 2015-11-02
Accepted : 2016-10-26
Published : 2016-11-21
Keywords:
Performance,
vitamin E,
Ascorbic acid,
broiler chicken,
immunity,
blood parameters,
betaine,
Abstract :
In order to investigate the effects of vitamins C and E and betaine on performance, immune response and blood parameters of broiler chickens, an experiment was conducted with completely randomized block design consisting of 6 treatments and 5 replicates with 30 chicks in each replicate. The groups were assigned to receive the treatment diets as follows: diet with no feed additive (control); diet supplemented with vitamin C; diet supplemented with vitamin E; diet supplemented with betaine; diet supplemented with vitamin E and betaine; diet supplemented with vitamin E, vitamin C and betaine. Body weight, feed intake, body weight gain, feed conversion ratio and mortality were determined over the course of the experiment. At 35 days of age, two birds per replicate were randomly selected and 2 ml blood sample was collected to measure plasma concentration of T3 and T4 hormones. IgM and IgG antibody response to SRBC were also determined at 28 days of age. Body weight, FI and FCR were not affected by different treatments over the course of the study. Broilers fed diet supplemented with vitamin C, vitamin E and betaine showed the highest survival percentage and production index as compared with the other treatments. But it was not significantly different with the control group. IgM and IgG antibody response to SRBC, hematocrit percentage and plasma concentration of T3 and T4 also were not affected by different dietary treatments. The results of this experiment showed that there were no significant differences among the treatments in the performance, immune response and blood parameters of broiler chicken.
References:
Cawthorn, D., Beers, K and Bottje, W.G. (2001). Electron transport chain defect and inefficient respiration may underlie pulmonary hypertension syndrome (ascites)-associated mitochondrial dysfunction in broilers. Poultry Science, 80: 474-484.
Closter, A.M., Van As, P., Groenen, M.A.M., Vereijken, A.L.J., Van Arendonk, J.A.M. and Bovenhuis, H. (2009). Genetic and phenotypic relationships between blood gas parameters and ascites-related traits in broilers. Poultry Science, 88: 483- 490.
Currie, R.J.W. (1999). Ascites in poultry: recent investigations. Avian Pathology, 28: 313-326.
Cueva, S., Sillau, H., Valenzuela, A. and Ploog, H. (1974). High altitude induced pulmonary hypertension and right heart failure in broiler chickens. Research Veterinary Science, 16: 370-374.
Coelho, M.B. and Mcnaughhton, J.L. (1995). Effect of composite vitamin supplementation on broilers. Applied Poultry Research, 4 (3): 219-299.
Daneshyar, M., Kermanshahi, H. and Golian, A. (2007). Changes of blood gases, internal organ weights and performance of broiler chickens with cold induced ascites. Journal of Animal and Veterinary Advances, 2: 729-735.
Decuypere, E., Buyse, J. and Buys, N. (2000). Ascites in broiler chickens: exogenous and endogenous structural and functional causal factors. World’s Poultry Science Journal, 56: 367-376.
Delhanty, J. and Solomon, J.B. (1966). The nature of antibodies to goat erythrocytes in the developing chicken. Immunology, 11: 103-113.
Deyhim, F. and Teeter, R.G. (1993). Dietary vitamin and/or trace mineral premix effects on performance, humeral mediated immunity and carcass composition of broilers during thermo neutral and high ambient temperature distress. Applied Poultry Research, 2: 347-355.
Enkvetchakul, B., Bottje, W., Anthony, N., Moore, R. and Huff, W. (1993). Compromised antioxidant status associated with ascites in broilers. Poultry Science, 72: 2272-2280.
Farooqi, A.G. Khan, M.A. Rabbani, M., Perviz, K. and Khan, J.A. (2005). Evaluation of Betaine and Vitamin C in Allevation of Heat Stress in broilers. International Journal of Agriculture and Biology, 5: 744-746.
Gross, W. (1988). Effect of ascorbic acid on antibody response of stressed and unstressed chickens. Avian Diseases, 32: 483-485.
Hassanzadeh, M., Buyse, J. and Decuypere, E. (2002). Further evidence for the involvement of cardiac- adrenergic receptors in right ventricle hypertrophy and ascites in broiler chickens. Avian Pathology, 31: 177-181.
Ivatury, R.R., Simon, R.J., Islam, S., Fueg, A., Rohman, M. and Stahl, W.M. (1996). A prospective randomized study of end points of resuscitation after major trauma: Global oxygen transport indices versus organ- specific gastric mucosal pH. Journal of the American College of Surgeons, 183:145-154.
Isakov, N., Feldmann, M and segel, S. (2005). The mechanism of modulation of humoral immuno responses after injection of mice with SRBC. Journal of Immunology, 128: 969-975.
Khajali, F., Asadi, K.E. and Zamani, M.A.K. (2006). Effect of vitamin and trace mineral withdrawal from finisher diets on growth performance and immune competence of broiler chickens. British Poultry Science, 47: 159-162.
Karaca, M., Johnson, E. and Lamont, S.J. (1999). genetic line and major histocompatibility complex effects on primary and secondary antibody responses to T-dependent and T-independent antigens. Poultry Science, 78: 1518-1525.
Ladmakhi, M.H., Buys, N., Dewil, E., Rahimi, G. and Decuypere. E. (1997). The prophylactic effect of vitamin C supplementation on broiler ascites incidence and plasma thyroid hormone concentration. Avian Pathology, 26: 33- 44.
Luger, D., Shinder, D., Rzepakovsky, V., Rusal, M. and Yahav, S. (2001). Association between weight gain, blood parameters, and thyroid hormones and the development of ascites syndrome in broiler chickens. Poultry Science, 80: 965-971.
Maiorka, A., Laurentiz, A.C., Santin, E., Araujo, L.F. and Macari, M. (2002). Dietary vitamin or mineral mix removal during the finisher period on broiler chicken performance. Journal of Applied Poultry Research, 11: 121-126.
McCormiek, D. B. (1994). Vitamins. In: Tietz textbook of clinical chemistry. McCormiek, D.B. and Green, H.L. editors. 2nd ed., Philadelphia: Sounders Company, pp: 1251-1295.
Mc Kee, J.S. and Harrison, P.C. (1995). Effect of supplemental ascorbic acid on the performance of the broiler chicken exposed to multiple concurrent stressors. Poultry Science, 74: 1772-1785.
Meimandipour, A., Hair-Bejo, M., Shuhaimi, M., Azhar, K., Soleimani, A.F., Rasti, B., et al. (2010). Gastrointestinal tract morphological alteration by unpleasure physical contact and modulating role of Lactobacillus in broiler. British Poultry Science, 51: 52-59.
Miller, S.M. (1991). Clinical chemistry, principles, procedures, correlations. 2nd ed., New York: Lippicott, pp: 215-235.
MINITAB. (2005). Teaching Statistics. MINITAB® French Release 14.
National Research Council (NRC). (1994). Nutrient requirements of poultry. National Academe Press. Washington. D.C.
Null, G. (2001). The antioxidant vitamin- vitamin c. WWW.Vitamin c foundaion.org. DOCREP/ARTICLE.
Pakdel, A. (2004). Genetic analysis of ascites-related traits in broilers. PhD thesis, Wageningen University. Wageningen, The Netherlands, pp: 133.
Puron, D., Santamaria, P. and Segura, J.C. (1994). Effect of Sodium Bicarbonate, Acetylsalicylic and Ascorbic acid on broiler performance in a tropical environment. Journal of Applied Poultry Research, 3: 141-145.
Sahin, K., Sahin, N., Onderci, M., Gursu, M.F. and Cikim, G. (2002). Optimal dietary concentration of chromium for alleviating the effect of heat stress on growth, carcass qualities, and some serum metabolites of broiler chickens. Biological Trace Element Research, 89: 53- 64.
SAS: Institute. (1990). SAS user, s Guide: Statistics, Version 6, 4th ed., SAS Inst. Inc. Cary, NC.
Tengerdy, R.P. (1990). The role of vitamin E in immune response and disease resistance. Annals of the New York Academy of Sciences, 587: 24-33.
