Evaluation of acute phase proteins in cows affected by Traumatic Reticuloperitonitis

Subject Areas : Veterinary Clinical Pathology

Ali Rezaei ¹ , A.P Rezaei Saber ² , افشین Davasaz Tabrizi ³

1 - Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
2 - Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
3 - Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran

Received: 2008-10-29 Accepted : 2009-01-19 Published : 2008-11-21

Keywords: Protein, Cow, peritonitis, reticulum,

Abstract :

     Traumatic Reticuloperitonitis is one of the most important diseases of the bovine digestive system which if diagnosed in early stage and before severe adhesions develop, more effective treatments leading to better prognosis could be under taken. In the course of accurate and timely diagnosis                            of these diseases, acute phase proteins of the affected animals were evaluated. For this purpose,                       blood samples were taken form jugular vein of 25 Holstein cows in which the presence of disease         clinically and confirmed with laparatomy and their sera separated. Blood sample were also collected from 20 healthy cows as the control group. After preparing serum sample, total protein haptoglobin, serum amyloid A and ceruloplasmin were measured in both groups. Total protein was determined by the Biuret method, haptoglobin and serum amyloid A by ELISA and ceruloplasmin through determination of the oxidative activity. Statistical analysis indicated significant difference in the amounts of total protein, haptoglobin and serum amyloid A between the two groups (p<0.05) but there was no significant difference in the amount of ceruloplasmin. This study demonstrated that was of haptoglobin and serum amyloid A could be beneficial n the diagnosis of bovine traumatic reticuloperitonitis.                

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