Biochemical variants among ovine Pasteurella multocida biovars isolated from East Azarbaijan province

Subject Areas : Veterinary Clinical Pathology

جلال Shayegh ¹ , هادی Keyvanfer ² , جلیل Dolgari Sharaf ³ , منصور Khakpour ⁴

1 - Department of Veterinary Medicine, Islamic Azad University, Shabestar Branch, Shabestar, Iran
2 - Microbiology Department, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Campus, Tehran, Iran
3 - Department of Veterinary Medicine, Islamic Azad University, Shabestar Branch, Shabestar, Iran
4 - Pathology Department, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran

Received: 2008-10-28 Accepted : 2009-01-19 Published : 2008-08-22

Keywords: East Azarbaijan, Pasteurella multocida, Sheep, Biochemical variants,

Abstract :

      In this study, biochemical variants of 24 ovine Pasteurella multocida isolates which were biotyped by microplate fermentation were studied. All isolates were taken from sheep with nasal discharge in East Azarbaijan province. Among the five identified biovars namely biovars 2, 3, 6, 7 and 11 only biovars 6 and 7 had biochemical variants. Manitol negative, manitol negative/ornitine decarboxylase negative and manitol negative maltose negative variants among biovar 6 and saccharose positive among biovar 7 are the characteristics of ovine P. multocida isolated from this province.

References:

1. جباری، ا.، اسماعیلی، ف.، صفی مرندی، م. و ﭘوربخش، س.ع. (١٣٨٠): مطالعه بیوتیپ و سروتیپ ﭘاستورلا مولتوسیدا جدا شده از طیور ایران، ﭘژوهش وسازندگی، دوره ٥۲، صفحات: ۶۴-۶۷.


2. زهرائی صالحی، ت. و شایق، ج. (١٣٨6): میکروب‌شناسی دامپزشکی و بیماری‌های میکروبی (بخش بیماری‌های باکتریائی) (ترجمه)،  تالیف: کویین وهمکاران، انتشارات دانشگاه تهران، صفحات: 74-56.


3. Blackall, P.J., Pahoff, J.L. and Bowles, R. (1997): Phenotypic characterization of Pasteurella multocida isolates from Australian pigs .Veterinary Microbiology, 57: 355–360.


4. Blackall, P.J., Pahoff, J.L., Marks, D., Fegan, N. and Morrow, C.J. (1995): Characterization of Pasteurella multocida isolates from fowl cholera outbreaks on seven turkey farms. Aust. Vet. J., 57: 382-387.

1. Davies, R.L. (2004): Genetic diversity among Pasteurella multocida strains of avian, bovine, ovine and porcine origin from England and Wales by comparative sequence analysis of the 16S rRNA gene. Microbiology, 150.Pt 12: 4199-210.

6. Fegan, N., Blackall, P.J. and Pahoff, J.L. (1995): Phenotypic characterization of Pasteurella multocida isolates from Australian poultry. Vet. Microbiol., 47: 281-286.


7. Harper, M., Boyce, J.D. and Adler, B. (2006): Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiol. Lett., 265: 1–10.


8. Mutters, R., Ihm, P., Pohl, S., Frederiksen, W. and Mannheim, W. (1985): Reclassification of the genus Pasteurella trevisan on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa. Int. J. Syst. Bacteriol., 35: 309–322.


9. Shayegh, J., Atashpaz, S. and Hejazi, M.S. (2008): Virulence genes profile and typing of ovine Pasteurella multocida. Asian Ani. Vet. Ad., 3(4): 206-13.


10. Townsend, K.M., O’Boyle, D. and Phan, T.T., et al. (1998): Acute septicaemic pasteurellosis in Vietnamese pigs. Vet. Microbiol., 63: 205-215.