Effect of Malva sylvestris hydroethanolic leaf extract on the healing of full-thickness, excisional skin wounds in the rat
Subject Areas :
Veterinary Clinical Pathology
mohammadreza Farahpour
1
,
saro Sedaghat
2
1 - استادیار گروه علوم درمانگاهی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.
2 - دانشجوی گروه علوم درمانگاهی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.
Received: 2015-05-22
Accepted : 2015-09-21
Published : 2015-05-22
Keywords:
Rat,
Malva sylvestris,
Hydroethanolic extract,
Excisional wound healing,
Abstract :
The healing of full-thickness wounds has always attracted attention due to disruption of the healing process that leads to prolonged recovery period. Therefore, the quest for finding clinically effective wound-healing agents is important in the medical management of full-thickness wounds. The present study was conducted to investigate the effects of Malva sylvestris hydroethanolic leaf extract on wound healing process in 54 male rats. Two standardized circular surgical full-thickness excisional wounds with a diameter of 7mm were created in the dorsal skin of vats in all three groups. The rats in the two treatment groups were treated with ointments containing 1.5% and 3% of the extract mixed with yellow soft paraffin, whereas the rats in the control group were only treated with yellow soft paraffin. Treatments were applied immediately after wounding and also for 16 consecutive days post-wounding. Wound-healing was assessed microscopically in terms of the initiation of neo-dermal tissue deposition and wound closure over a 16-day period. Significant increase in wound contraction rate was seen in the treatment groups, especially the 3% group, compared to the control group (P<0.05). Moreover, the inflammatory cell score showed a decrease, whereas new vessel formation, fibroblast distribution, collagen production and epidermis thickness increased in Malva sylvestris hydroethanolic leaf extract-treated animals. These results suggest that the topical application of Malva sylvestris hydroethanolic leaf extract has beneficial effects on full-thickness excisional wound healing.
References:
· Beldon, P. (2010). Basic science of wound healing. Surgery (Oxford), 28: 409.
· Dehkordi, N . (2003). Iranian herbal pharmacopoeia. Tehran: Ministry of Health, 206-11.
· Dissemond, J., Goos, M. and Wagner, S.N. (2002). The role of oxidative stress in the pathogenesis and therapy of chronic wounds. Hautarzt, 53: 718-23.
· Esteves, P.F., Sato, A., Esquibel, M.A., Campos-Buzzi, F.D., Meira, A.V. and Cechinel-Filho, V. (2009). Antinociceptive activity of Malva sylvestris L. Latin American Journal of Pharmacy, 28(3): 454-6.
· Eseyin, O.A., Ebong, P., Ekpo, A., Igboasoiyi, A. and Oforah, E. (2007). Hypoglycemic effect of the seed extract of Telfairia occidentalis in rat. Pakistan Journal of Biological Sciences, 10(3): 498-501.
· Farahpour, M.R., Mirzakhani, N., Doostmohammadi, J. and Ebrahimzadeh, M. (2015). Hydroethanolic Pistacia atlantica hulls extract improved wound healing process; evidence for mast cells infiltration, angiogenesis and RNA stability. International Journal of Surgery, 17: 88-98.
· Folkman, J. (1995). Angiogenesis in cancer, vascular, rheumatoid and other disease. Natural Medicine, 1(1): 27-31
· Gasparetto, J.C., Martins, C.A.F., Hayashi, S.S., Otuky, M.F. and Pontarolo, R. (2012). Ethnobotanical and Scientific aspects of Malva Sylvestris L: a millennial herbal medicine. Journal of Pharmacy and Pharmacology, 64(2): 172-189.
· Lim, T. K. (2014). Malva sylvestris. In Edible Medicinal and Non Medicinal Plants. pp: 395-404.
· McDaniel, J.C., Massey, K. Nicolaou, A. (2011). Fish oil supplementation alters levels of lipid mediators of inflammation in microenvironment of acute human wounds. Wound Repair Regeneration, 19(2): 189-200.
· Mihaylova, D., Popova, A., Denkova, R., Alexieva, I., Krastanov, A. (2015). in vitro antioxidant and antimicrobial activity of extracts of Bulgarian Malva sylvestris. First National Conference of Biotechnology, Sofi, 100(4): 41-48.
· Ozay, Y., Ozyurt, S., Guzel, S., Cimbiz, A., Olgun, E.G. and Cayci, M. (2010). Effects of Equisetum arvense Ointment on Dermal Wound Healing in Rats. Wounds, 22(10): 261
· Ozkan, A.M. and Kutselm, O. (2005). Anti-ulcerogenic activity of some plants used in folk medicine of pinnarbasi. Journal of Ethnopharmacology, 101: 313-318.
· Pirbalouti, A.G., Yousefi, M., Heshmetollah, N., Karimi, I. and Koohpayeh, A. (2009). Evaluation of burn healing properties of Arnehia euchroma and Malva sylvestris. Electronic Journal of Biology, 5: 62-66.
· Pirbalouti, A.G. and Koohpyeh, A. (2010). Wound healing activity of extracts of Malva sylvestris and Stachys lavandulifolia. International Journal of Biology, 3(1): 174.
· Prudente, A.S., Loddi, A.M., Duarte, M.R., Santos, A.R., Pochapski, M.T., Pizzolatti, M.G., et al. (2013). Pre-clinical anti-inflammatory aspects of a cuisine and medicinal millennial herb: Malva sylvestris L. Food and Chemical Toxicology, 58: 324-331.
· Razavi, S.M., Zarrini, G., Molavi, G. and Ghasemi, G. (2011). Bioactivity of Malva sylvestris L., a medicinal plant from Iran. Iranian journal of basic medical sciences, 14(6): 574.
· Sleiman, N.H. and Daher, C.F. (2009). Malva sylvestris water extract: a potential anti-inflammatory and anti-ulcerogenic remedy. Planta Medica, 75(09), PH10.
· Shetty, S., Udupa, S. and Udupa, L. (2008). Evaluation of antioxidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn in rats. Evidence-Based Complementary and Alternative Medicine, 5(1): 95-101.
· Zargari, A. (1992). Medicinal Plants. 5th ed., Iran: Tehran, University Publication, pp:346-352