Assessment of oxidative stress indexes and BCS in clinical mastitis cows in comparison with healthy cows
Subject Areas :
Veterinary Clinical Pathology
Maryam Karimi Dehkordi
1
,
Seyedeh Ommolbanin Ghasemian
2
*
1 - Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
2 - Assistant Professor, Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran.
Received: 2021-11-30
Accepted : 2022-04-25
Published : 2022-05-22
Keywords:
antioxidant enzyme,
dairy cow,
Oxidative stress,
clinical mastitis,
Abstract :
Mastitis, as one of the most important diseases in cattle, is the cause of the greatest economic loss in the dairy industry. The occurrence of mastitis is associated with the development of immune responses and an increase in the level of reactive oxygen species (ROS). The production of high amounts of ROS and the lack of optimal amounts of antioxidant compounds are associated with oxidative stress. The aim of this study was to evaluate the status of oxidative stress indices in cows with clinical mastitis. In this study, two groups of cows were studied: healthy cows and cows with clinical mastitis. The body condition score (BCS) of the cows was assessed at the time of blood sampling.Total antioxidant capacity and selenium concentration were significantly higher in healthy cows than sick cows. Healthy cows had lower malondialdehyde concentrations than sick cows, although this difference was not statistically significant. When the cows were divided into three groups according to body score, the amount of glutathione peroxidase in cows with high body scores was significantly lower than the other two groups. The amount of malondialdehyde and the number of milk somatic cells in cows with high and moderate body scores were significantly higher than the other group.The results show that there are changes in the antioxidant defense of cows with mastitis that lead to oxidative damage, so the use of antioxidants is necessary to control mastitis. Obese cows are also more sensitive to oxidative stress.
References:
Alharthi, A., Zhou, Z., Lopreiato, V., Trevisi, E. and Loor, J.J. (2018). Body condition score prior to parturition is associated with plasma and adipose tissue biomarkers of lipid metabolism and inflammation in Holstein cows. Journal of Animal Science and Biotechnology, 9(1): 1-12.
Allison, R.D. and Laven, R.A. (2000). Effect of vitamin E supplementation on the health and fertility of dairy cows: a review. Veterinary Record, 147(25): 703-708.
Andrei, S.M.S.R.D., Matei, S., Rugină, D., Bogdan, L. and Ştefănuţ, C. (2016). Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow’s milk. Czech Journal of Animal Science, 61(9): 407-413.
Atakisi, O., Oral, H., Atakisi, E., Merhan, O., Pancarci, S.M., Ozcan, A., et al. (2010). Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Research in Veterinary Science, 89(1): 10-13.
Bernabucci, U., Ronchi, B., Lacetera, N. and Nardone, A. (2005). Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science, 88(6): 2017-2026.
Dandona, P., Aljada, A. and Bandyopadhyay, A. (2004). Inflammation: the link between insulin resistance, obesity and diabetes. Trends in Immunology, 25(1): 4-7.
Darbaz, I., Salar, S., Sayiner, S., Baştan, İ., Ergene, O. and Baştan, A. (2019). Evaluation of milk glutathione peroxidase and superoxide dismutase levels in subclinical mastitis in Damascus goats. Turkish Journal of Veterinary and Animal Sciences, 43(2): 259-263.
El-Deeb, W.M. (2013). Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers. Journal of Zhejiang University Science B, 14(1): 33-39.
Ellah, M.R.A. (2013). Role of free radicals and antioxidants in mastitis. Journal of Advanced Veterinary Research, 3(1): 1-7.
El-Sharawy, M.E., Mashaly, I.M., Atta, M.S., Kotb, M. and El-Shamaa, I.S. (2019). Influence of body condition score on blood metabolites and oxidative stress in pre-and post-calving of Friesian dairy cows in Egypt. Slovenian Veterinary Research, 56(Suppl. 22): 209-217.
Ghasemian, K.O., Safi, S., Rahimi froushani, A. and Bolourchi, M. (2011). Study of the relationship between oxidative stress and subclinical mastitis in dairy cattle; short paper. Iranian Journal of Veterinary Research, Shiraz University, 12(4): 37
Jhambh, R., Dimri, U., Gupta, V.K. and Rathore, R. (2013). Blood antioxidant profile and lipid peroxides in dairy cows with clinical mastitis. Veterinary World, 6(5): 271.
Karimi-Dehkordi, M., Kadivar, A. and Taktaz Hafshejani, T. (2016). Critical Thresholds of Nonesterified Fatty Acids and β-hydroxybutyrate in Transition Dairy Cows for Prediction of First Service Conception Rate. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(2): 191-195.
Karimi-Dehkordi, M., Salehi, N. and Banimehdi, P. (2021). Comparison of serum oxidative status in healthy Arabian and Dareshoor horses. Journal of Veterinary Clinical Pathology, 15(1): 29-39. [In Persian]
Keaney Jr, J.F., Larson, M.G., Vasan, R.S., Wilson, P.W., Lipinska, I., Corey, D., et al. (2003). Obesity and systemic oxidative stress: clinical correlates of oxidative stress in the Framingham Study. Arteriosclerosis, Thrombosis, and Vascular Biology, 23(3): 434-439.
Khalili, M., Chamani, M., Amanlou, H., Nikkhah, A., Sadeghi, A.A., Dehkordi, F.K., et al. (2020). The effect of feeding inorganic and organic selenium sources on the hematological blood parameters, reproduction and health of dairy cows in the transition period. Acta Scientiarum. Animal Sciences, 42(1): 1-10.
Kizil, O., Akar, Y.A.Ş.A.R., Saat, N., Kizil, M. and Yuksel, M. (2007). The plasma lipid peroxidation intensity (MDA) and chain-breaking antioxidant concentrations in the cows with clinic or subclinic mastitis. Revue de Medecine Veterinaire, 158(11): 529-533.
Kuloglu, M., Atmaca, M., Tezcan, E., Ustundag, B. and Bulut, S. (2002). Antioxidant enzyme and malondialdehyde levels in patients with panic disorder. Neuropsychobiology, 46(4): 186-189.
Laubenthal, L., Ruda, L., Sultana, N., Winkler, J., Rehage, J., Meyer, U., et al. (2017). Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows. Journal of Dairy Science, 100(6): 4976-4986.
Malik, T.A., Mohini, M., Mir, S.H., Ganaie, B.A., Singh, D., Varun, T.K., et al. (2018). Somatic cells in relation to udder health and milk quality-a review. Journal of Animal Health and Production, 6(1): 18-26.
Martins, S.A.M., Martins, V.C., Cardoso, F.A., Germano, J.H., Rodrigues, M.C., Duarte, C.M., et al. (2019). Biosensors for on-farm diagnosis of mastitis. Frontiers in Bioengineering and Biotechnology, 7 (1): 186.
Matei, S.T., Groza, I., Bogdan, L., Ciupe, S., Fiţ, N. and Andrei, S. (2011). Correlation between mastitis pathogenic bacteria and Glutathione peroxidase activity in cows’ milk. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 1(68): 221-225.
Mehdi, Y. and Dufrasne, I. (2016). Selenium in cattle: a review. Molecules, 21(4): 1-14.
Miller, N.J., Rice-Evans, C., Davies, M.J., Gopinathan, V. and Milner, A. (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science, 84(4): 407-412.
Morrow, J.D. (2003). Is oxidant stress a connection between obesity and atherosclerosis? Arteriosclerosis, Thrombosis, and Vascular Biology, 23(3): 368-370.
O'Boyle, N., Corl, C.M., Gandy, J.C. and Sordillo, L.M. (2006). Relationship of body condition score and oxidant stress to tumor necrosis factor expression in dairy cattle. Veterinary Immunology and Immunopathology, 113(3-4): 297-304.
Omur, A., Kirbas, A., Aksu, E., Kandemir, F., Dorman, E., Kaynar, O., et al. (2016). Effects of antioxidant vitamins (A, D, E) and trace elements (Cu, Mn, Se, Zn) on some metabolic and reproductive profiles in dairy cows during transition period. Polish Journal of Veterinary Sciences, 19(4): 697-706.
Rehman, S.B., Hussain, I. and Rashid, S.M. (2017). Assessment of antioxidant profile in subclinical and clinical mastitis in dairy cattle. Journal of Entomology and Zoology Studies, 5(6): 1022-1025.
Vasiľ, M., Elečko, J., Farkašová, Z. and Bíreš, J. (2009). The reduction on the occurrence of mastitis in dairy herd using the innovation of housing conditions, sanitary of milk storage and applying the therapy of mastitis during the lactation. Folia Veterinaria, 53(2): 186-189.
Vendemiale, G., Grattagliano, I., Caraceni, P., Caraccio, G., Domenicali, M., Dall'Agata, M., et al. (2001). Mitochondrial oxidative injury and energy metabolism alteration in rat fatty liver: effect of the nutritional status. Hepatology, 33(4): 808-815.
Wu, J., Liu, J. and Wang, D. (2020). Effects of body condition on the insulin resistance, lipid metabolism and oxidative stress of lactating dairy cows. Lipids in Health and Disease, 19(1): 1-7.
Yang, S., Zhu, H., Li, Y., Lin, H., Gabrielson, K. and Trush, M.A. (2000). Mitochondrial adaptations to obesity-related oxidant stress. Archives of Biochemistry and Biophysics, 378(2): 259-268.
Zigo, F., Elecko, J., Vasil, M., Ondrasovicova, S., Farkasova, Z. and Malova, J. (2019). The occurrence of mastitis and its effect on the milk malondialdehyde concentrations and blood enzymatic antioxidants in dairy cows. Veterinární Medicína, 64(10): 423-432.
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Alharthi, A., Zhou, Z., Lopreiato, V., Trevisi, E. and Loor, J.J. (2018). Body condition score prior to parturition is associated with plasma and adipose tissue biomarkers of lipid metabolism and inflammation in Holstein cows. Journal of Animal Science and Biotechnology, 9(1): 1-12.
Allison, R.D. and Laven, R.A. (2000). Effect of vitamin E supplementation on the health and fertility of dairy cows: a review. Veterinary Record, 147(25): 703-708.
Andrei, S.M.S.R.D., Matei, S., Rugină, D., Bogdan, L. and Ştefănuţ, C. (2016). Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow’s milk. Czech Journal of Animal Science, 61(9): 407-413.
Atakisi, O., Oral, H., Atakisi, E., Merhan, O., Pancarci, S.M., Ozcan, A., et al. (2010). Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Research in Veterinary Science, 89(1): 10-13.
Bernabucci, U., Ronchi, B., Lacetera, N. and Nardone, A. (2005). Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. Journal of Dairy Science, 88(6): 2017-2026.
Dandona, P., Aljada, A. and Bandyopadhyay, A. (2004). Inflammation: the link between insulin resistance, obesity and diabetes. Trends in Immunology, 25(1): 4-7.
Darbaz, I., Salar, S., Sayiner, S., Baştan, İ., Ergene, O. and Baştan, A. (2019). Evaluation of milk glutathione peroxidase and superoxide dismutase levels in subclinical mastitis in Damascus goats. Turkish Journal of Veterinary and Animal Sciences, 43(2): 259-263.
El-Deeb, W.M. (2013). Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers. Journal of Zhejiang University Science B, 14(1): 33-39.
Ellah, M.R.A. (2013). Role of free radicals and antioxidants in mastitis. Journal of Advanced Veterinary Research, 3(1): 1-7.
El-Sharawy, M.E., Mashaly, I.M., Atta, M.S., Kotb, M. and El-Shamaa, I.S. (2019). Influence of body condition score on blood metabolites and oxidative stress in pre-and post-calving of Friesian dairy cows in Egypt. Slovenian Veterinary Research, 56(Suppl. 22): 209-217.
Ghasemian, K.O., Safi, S., Rahimi froushani, A. and Bolourchi, M. (2011). Study of the relationship between oxidative stress and subclinical mastitis in dairy cattle; short paper. Iranian Journal of Veterinary Research, Shiraz University, 12(4): 37
Jhambh, R., Dimri, U., Gupta, V.K. and Rathore, R. (2013). Blood antioxidant profile and lipid peroxides in dairy cows with clinical mastitis. Veterinary World, 6(5): 271.
Karimi-Dehkordi, M., Kadivar, A. and Taktaz Hafshejani, T. (2016). Critical Thresholds of Nonesterified Fatty Acids and β-hydroxybutyrate in Transition Dairy Cows for Prediction of First Service Conception Rate. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(2): 191-195.
Karimi-Dehkordi, M., Salehi, N. and Banimehdi, P. (2021). Comparison of serum oxidative status in healthy Arabian and Dareshoor horses. Journal of Veterinary Clinical Pathology, 15(1): 29-39. [In Persian]
Keaney Jr, J.F., Larson, M.G., Vasan, R.S., Wilson, P.W., Lipinska, I., Corey, D., et al. (2003). Obesity and systemic oxidative stress: clinical correlates of oxidative stress in the Framingham Study. Arteriosclerosis, Thrombosis, and Vascular Biology, 23(3): 434-439.
Khalili, M., Chamani, M., Amanlou, H., Nikkhah, A., Sadeghi, A.A., Dehkordi, F.K., et al. (2020). The effect of feeding inorganic and organic selenium sources on the hematological blood parameters, reproduction and health of dairy cows in the transition period. Acta Scientiarum. Animal Sciences, 42(1): 1-10.
Kizil, O., Akar, Y.A.Ş.A.R., Saat, N., Kizil, M. and Yuksel, M. (2007). The plasma lipid peroxidation intensity (MDA) and chain-breaking antioxidant concentrations in the cows with clinic or subclinic mastitis. Revue de Medecine Veterinaire, 158(11): 529-533.
Kuloglu, M., Atmaca, M., Tezcan, E., Ustundag, B. and Bulut, S. (2002). Antioxidant enzyme and malondialdehyde levels in patients with panic disorder. Neuropsychobiology, 46(4): 186-189.
Laubenthal, L., Ruda, L., Sultana, N., Winkler, J., Rehage, J., Meyer, U., et al. (2017). Effect of increasing body condition on oxidative stress and mitochondrial biogenesis in subcutaneous adipose tissue depot of nonlactating dairy cows. Journal of Dairy Science, 100(6): 4976-4986.
Malik, T.A., Mohini, M., Mir, S.H., Ganaie, B.A., Singh, D., Varun, T.K., et al. (2018). Somatic cells in relation to udder health and milk quality-a review. Journal of Animal Health and Production, 6(1): 18-26.
Martins, S.A.M., Martins, V.C., Cardoso, F.A., Germano, J.H., Rodrigues, M.C., Duarte, C.M., et al. (2019). Biosensors for on-farm diagnosis of mastitis. Frontiers in Bioengineering and Biotechnology, 7 (1): 186.
Matei, S.T., Groza, I., Bogdan, L., Ciupe, S., Fiţ, N. and Andrei, S. (2011). Correlation between mastitis pathogenic bacteria and Glutathione peroxidase activity in cows’ milk. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 1(68): 221-225.
Mehdi, Y. and Dufrasne, I. (2016). Selenium in cattle: a review. Molecules, 21(4): 1-14.
Miller, N.J., Rice-Evans, C., Davies, M.J., Gopinathan, V. and Milner, A. (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science, 84(4): 407-412.
Morrow, J.D. (2003). Is oxidant stress a connection between obesity and atherosclerosis? Arteriosclerosis, Thrombosis, and Vascular Biology, 23(3): 368-370.
O'Boyle, N., Corl, C.M., Gandy, J.C. and Sordillo, L.M. (2006). Relationship of body condition score and oxidant stress to tumor necrosis factor expression in dairy cattle. Veterinary Immunology and Immunopathology, 113(3-4): 297-304.
Omur, A., Kirbas, A., Aksu, E., Kandemir, F., Dorman, E., Kaynar, O., et al. (2016). Effects of antioxidant vitamins (A, D, E) and trace elements (Cu, Mn, Se, Zn) on some metabolic and reproductive profiles in dairy cows during transition period. Polish Journal of Veterinary Sciences, 19(4): 697-706.
Rehman, S.B., Hussain, I. and Rashid, S.M. (2017). Assessment of antioxidant profile in subclinical and clinical mastitis in dairy cattle. Journal of Entomology and Zoology Studies, 5(6): 1022-1025.
Vasiľ, M., Elečko, J., Farkašová, Z. and Bíreš, J. (2009). The reduction on the occurrence of mastitis in dairy herd using the innovation of housing conditions, sanitary of milk storage and applying the therapy of mastitis during the lactation. Folia Veterinaria, 53(2): 186-189.
Vendemiale, G., Grattagliano, I., Caraceni, P., Caraccio, G., Domenicali, M., Dall'Agata, M., et al. (2001). Mitochondrial oxidative injury and energy metabolism alteration in rat fatty liver: effect of the nutritional status. Hepatology, 33(4): 808-815.
Wu, J., Liu, J. and Wang, D. (2020). Effects of body condition on the insulin resistance, lipid metabolism and oxidative stress of lactating dairy cows. Lipids in Health and Disease, 19(1): 1-7.
Yang, S., Zhu, H., Li, Y., Lin, H., Gabrielson, K. and Trush, M.A. (2000). Mitochondrial adaptations to obesity-related oxidant stress. Archives of Biochemistry and Biophysics, 378(2): 259-268.
Zigo, F., Elecko, J., Vasil, M., Ondrasovicova, S., Farkasova, Z. and Malova, J. (2019). The occurrence of mastitis and its effect on the milk malondialdehyde concentrations and blood enzymatic antioxidants in dairy cows. Veterinární Medicína, 64(10): 423-432.
