Evaluation of polyclonal antibody production level against Listeria monocytogenes in animal model

Subject Areas : Veterinary Clinical Pathology

Yasin Bagheri ¹ , Mansour Khakpour ² , Hamid Zihag ³ , Alireza Barati ⁴

1 - Young Researchers and Elite Club, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
2 - Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
3 - D.V.M. Graduate, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
4 - Young Researchers and Elite Club, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

Received: 2018-11-08 Accepted : 2019-11-25 Published : 2019-11-22

Keywords: Listeria monocytogenes, polyclonal antibodies, animal model,

Abstract :

Listeria monocytogenes is a short, gram-positive, spore-free bacillus and facultative intracellular parasite, which is transmitted through contaminated vegetables, milk, cheese and meat to humans. Immunoglobulin Y (IgY) obtained from the egg yolk of immunized hens is a great and cheap source of polyclonal antibodies. These antibodies can be an appropriate replacement for antibodies produced in other laboratory animals. In recent years, IgY has been used widely in medical research for diagnosis, prevention and treatment of diseases. In current study, 12 Hy- line laying hens were divided into 2 groups of control (2 hens) and treatment (10 hens) and the treatment group was immunized by triple injection of Listeria monocytogenes antigens once a week. Then, after collecting the eggs and measuring total protein and yolk globulins at weeks 9,10 and 11 after the first injection, it was observed that there was no significant difference in both parameters at 9 and 10 weeks, while they showed a significant difference compared to the control group (p < /em><0.05). However, the amount of globulin and total protein of yolk in the eleventh week was significantly reduced although no significant difference was observed with the controls. Accordingly, it could be concluded that immunization of hens by antigens prepared from Listeria monocytogenes resulted in increased yolk globulin levels in the ninth and tenth weeks after injection therefore egg yolk can be used as a source for producing polyclonal antibodies against Listeria monocytogenes.         

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