Effects of selenium nanoparticles and sodium selenite on expression of adiponectin gene in placenta of pregnant ewes.

Subject Areas : Veterinary Clinical Pathology

Pedram Moayeri ¹ , Gholam ali Kojouri ² , Afshin Jafari dehkordi ³ , Ali mohammad Ahadi ⁴ , Mahsa Abolfazlzadeh ⁵

1 - Shahrekord UnivePh.D. Graduate of Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran.rsity
2 - Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran.
3 - Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran.
4 - Assistant Professor, Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran.
5 - Graduate of Nursing, Astara Branch, Islamic Azad University, Astara, Iran.

Received: 2018-02-15 Accepted : 2019-07-30 Published : 2019-11-22

Keywords: Sheep, placenta, Sodium selenite, Adiponectin, selenium nanoparticles,

Abstract :

In recent years, special attention has been paid to insulin resistance mediators, such as adiponectin, that is produced and expressed during conception in placental tissue, thereby inducing its effects on the growing embryo and the health of the mother.In this study, the effect of selenium nanoparticles and sodium selenite on transcription of placental adiponectin gene was investigated for the first time during the transitional period. For this purpose, 20 pregnant ewes were selected randomly. During the 10 days leading up to birth, oral administration of selenium nanoparticles (Se NPs) with dosages of 0.05 and 0.10 mg/kg B.W. and sodium selenite with dosage of 0.1 mg/kg B.W. was performed. At the same time, the control group received equal volume of distilled water. At parturition, placental samples were collected and the transcription rate of adiponectin gene was determined by using RT-PCR based on a comparison assay of 2-ΔΔCt.The results showed that selenium supplementation in pregnant ewes leads to a significant reduction in adiponectin gene transcription rate and the suppressive role of selenium nanoparticles was significantly superior to sodium selenite.

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