Protective effects of nettle (Urtica dioica) extract against acute kidney injury induced by gentamycin in the rat

Subject Areas : Veterinary Clinical Pathology

mahdieh hajjavadi ¹ , akram eidi ² , سید پژمان Mortazavi ³

1 - MSc Graduate, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Professor, Department of Physiology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Associate Professor, Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Received: 2017-03-04 Accepted : 2017-04-10 Published : 2017-11-22

Keywords: Gentamicin, Rat, Urtica dioica, Acute renal injury,

Abstract :

Aminoglycosides are often used in combination with beta-lactam antibiotics and have a rapid bactericidal effect, are available at an affordable cost and have less incidence of resistance, making them a drug of choice for treatment of several life-threatening infections. However, the nephrotoxic effects of aminoglycosides prevent their long term use. The use of herbal extracts in order to decrease injuries of injurious materials has long been considered. The present study was conducted in order to investigate the protective effects of nettle (Urtica dioica) extract against gentamicin induced kidney injuries in the rat. Forty five male Wistar rats were divided into 9 groups consisting of: 1-healthy control group, 2- negative control group that received tween 20 (extract solvent), 3- patient control group which received onlygentamicin at 100 mg/kg, experimental healthy groups 4-6 which received nettle extract at 50, 100 and 200 mg/kg and patient experimental groups 7-9 which received nettle extract along with gentamicin at 100 mg/kg. At the end of the experiment (28 days), blood samples were obtained, and the kidneys were removed for histopathologic investigations. The results showed that gentamicin alone induced renal tissue damage and significantly increased the serum levels of creatinine and urea (p<0.05). However, administration of nettle extract accompanied with gentamicin decreased these markers significantly (p<0.05). Also histologic results indicated improvement of renal tissue structures during treatment with nettle extract.  It is concluded that nettle could ameliorate nephrotoxicity induced by gentamicin.  

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