Investigation of antibacterial properties and chemical essential oil components of Thymus Lancifolious Zagheh area(Lorestan province)

Subject Areas : Journal of Plant Ecophysiology

K. Karami ¹ , A. Heidari Jamshidi ² , A. Ariapour ³

1 - دانشجوی کارشناسی ارشد دانشگاه آزاد اسلامی واحد بروجرد،
2 - کارشناس ارشد زراعت و اصلاح نباتات سازمان جهاد کشاورزی استان لرستان
3 - عضو هیئت علمی دانشگاه آزاد اسلامی واحد بروجرد

Received: 2014-05-28 Accepted : 2014-05-28 Published : 2013-08-01

Keywords:

Abstract :

Lamiaceae family has about 200 genus and 4000 species which one of them is Thymus. Majority of this family have essence which utilize in medical, toiletry, hygienic and nutrient. Thymus genuses have many species which propagating in many provinces. The aim of this study was recognition and assessing of component in shoot structure of Thymus lancifolious and investigation of antibacterial of the essential oil on the species. Samples were collected in flowering stage in Gret of Lorestan province, Iran, in 2011. The samples were shadow dried and extracted its components by Clevenger device as Hydrodistilation method. Then anatomical properties were studied by painting and using bismarc brown and methyl green stains. After producing essences, kind of components and percent of essential chemical components recognized and separated completely by using GC and GC/MS devices. Plate sink was used to investigate of antibacterial effects of  the plant extract on some g+ and g-pathogen bacteria such as Staphylocococus Aureus, Escherichia Coli, Pseudomonas aeroginosa and β.streoptocococs. Results showed that the species has the same anatomical properties with other spices such as T. eriocalyx and T. kotschyanus in Lorestan. According to Retention volume, Retention time, Kovats retention index and comparison this parameters with standards components, results showed that the essential oil of this species has 45 components (95.85%  of all components). The main constituents include Cymol (3.26%), Carvacrol (52.34%), Gamma-terpinene (10.86) and Thymol (16.43%) is. The results of this research with other researches in terms existing main compounds of the essential oil were the same but have different, That this difference might be caused by environmental factors or methods of harvesting that harvest time on essential oil biosynthesis, location, stage of plant growth, climate change and the regional factors, including them. Also The results showed that the antimicrobial effect the extracts have antibacterial effects of species in.