Investigation on the effect of seed priming with rhizobium bioinoculant and Plant Growth Promoting Rhizobacteria on chlorophyll, nutrients and grain yield in lentil under rainfed condition

Subject Areas : Journal of Plant Ecophysiology

Alireza Seyyed-Moradi ¹ , Afshin Mozaffari ²

1 - Masters student, Agronomy and plant breeding department, Ilam branch, Islamic Azad university, Iran
2 - Assistant Professor, Agronomy and plant breeding department, Ilam branch, Islamic Azad university, Iran

Received: 2018-11-12 Accepted : 2019-05-29 Published : 2020-06-21

Keywords: Azotobacter, Lentil, Azospirillum, Pseudomonas, Rhizobium,

Abstract :

To study the effect of seed priming with rhizobium bioinoculant and Plant Growth Promoting Rhizobacteria on chlorophyll, nutrients and grain yield in lentil rainfed, a factorial experiment was carried out in a randomized complete block design (RCBD) with four replicates in Karazan districts of Sirvan located in Ilam province during 2016-2017. Experimental treatments include the application of PGPR consists of five levels: inoculation seed with Azotobacter, Azospirillum, Pseudomonas individually, inoculation of seeds with mixed PGPR (Azotobacter + Azospirillum + Pseudomonas) and non-using of PGPR, and rhizobium bacteria factor at two levels: seed inoculation with rhizobium and non-use of Rhizobium. Simple and interaction effects of Rhizobium bacteria and PGPR bacteria application on all studied traits (except for interaction effect of experimental factors on grain yield and chlorophyll b content) showed highly significant (P <0.01). In general, the results of the experiment showed that the use of Rhizobium bacteria with PGPR bacteria, in particular, combined consumption treatment (Azotobacter + Azospirillum + pseudomonas), increased the grain yield, nitrogen, phosphorus and potassium, and chlorophyll a, b and a+b contents of leaf compared to Non-use rhizobium and PGPR bacteria. With regard to synergistic effects of Rhizobium and PGPR bacteria in improving the growth and grain yield of lentil plant, it is recommended to use the combination of Rhizobium and PGPR bacteria in the inoculum formulation used under arid and dry land areas.

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