Relation of water deficit stress and biofertilizer on some of antioxidant enzymes activity and role them on grain yield variation of barley (Hordeum vulgare)
Subject Areas : Journal of Plant Ecophysiology
1 - استادیار گروه زراعت و اصلاح نباتات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران
Keywords: Water deficit, Biofertilizer, superoxide dismutase, Glutathione peroxidase,
Abstract :
To evaluate the qualitative traits of barley in response to biofertilizers (Azotobacter, Pseudomonas and Azospirillum) with affected by different regimes of irrigation the experiment was carried out in Karaj Azad University research field in 2013 with split plot based on Completely Randomize Block Design with four replications. In this experiment irrigation treatments were in main plots with three levels which interrupt of irrigation at 80, 65 and 50% of field capacity (normal irrigation), (35 and 50 percent of humidity discharge) and sub plots contained seed inoculation with bacteria such as |inoculation with Azospirillum lipoferum, Azotobacter chrococcum, Pseudomonas putida, Pseudomonas×Azotobacter, Pseudomonas×Azospirillum, Azotobacter×Azospirillum | and control (un inoculation) in seven levels. The results of analysis of variance showed that biofertilizer had significant effect on antioxidant enzymes at 1% probability. The activity of antioxidant enzymes affected with biofertilizers were increased at water deficit condition which Azospirillum lipoferum caused increasing about 16.7% and 21.4% and Pseudomonas×Azospirillum caused increasing about 18.2% and 25.9% for superoxide dismutase and glutathione peroxidase in irrigation at 50% of field capacity, respectively than control. Yield showed low decrease in irrigation at 50% of field capacity than normal irrigation such as yield decreasing in irrigation at 50% of field capacity was only about 13.2% with affected by Azospirillum than normal irrigation. The data showed that biofertilizer have efficient role on barley yield at water deficit condition.
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