The effect of diets supplemented with vitamins C and E on WBC fluctuations of Acipenser ruthenus
Subject Areas : New Technologies in Aquaculture DevelopmentKeywords: vitamin E, WBC, Acipenser ruthenus, Sterlet, Keywords: Vitamins C,
Abstract :
Abstract[1]This study was conducted to determine the different levels of dietary vitamin C and vitamin E on WBC fluctuations of sterlet (Acipenser ruthenus) in Dr. Dadman International Sturgeon Research Institute. Nine diets supplemented with a combination of 0, 100 and 400 mg/kg vitamin C, L-ascorbyl-2-polyphosphate (APP), and 0, 100 and 400 mg/kg vitamin E, D-alpha- tocopherol, were fed to sterlet in 2 replicates for 15 weeks. Fifteen fish with the average weight of 350.92±14.28 were distributed to each of 18 tanks after adaptation with experimental diet. The fish were fed 3% of body weight per day. Blood samples were obtained from three fish of each tank at the end of the experiment. The results of blood samples at the end of 15th week revealed a significant difference between WBC and monocytes, lymphocytes and neutrophils (P<0.05).The highest and the lowest WBC count was observed in fish fed with diets containing 100 mg kg1- vitamin E and 400 mg kg1- vitamin C (diet 6) and fish fed with 400 mg kg1- vitamin E and 400 mg kg1- vitamin C (diet 9), respectively. Also, the highest monocytes, lymphocytes and neutrophils were observed in fish fed with diets containing 0 mg kg1- vitamin E and 0 mg kg1- vitamin C (diet 1), 0 mg kg1- vitamin E and 100 mg kg1- vitamin C (diet 2) and 400 mg kg1- vitamin E and 400 mg kg1- vitamin C (diet 9), respectively. On the other hand, the lowest monocytes, lymphocytes and neutrophils were observed in fish fed with diets containing 100 mg kg1- vitamin E and 0 mg kg1- vitamin C (diet 4), 400 mg kg1- vitamin E and 400 mg kg1- vitamin C (diet 9) and 0 mg kg1- vitamin E and 100 mg kg1- vitamin C (diet 2), respectively.
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