The effects of anti-streptococci vaccine by bath method on some blood parameters of rainbow trout(Oncorhynchus mykiss)

Subject Areas : New Technologies in Aquaculture Development

Siamak Yousefi Siahkalroodi ¹ (Department of Biology, Faculty of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran)
Mohamad Mazandarani ² (Department of Fisheries, Faculty of Environment and Fisheries, Gorgan University of agricultural sciences and natural resources, Gorgan, Iran)
Raad Abdolyameh Habib ³ (Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran)
Parastoo Mohebi Derakhsh ⁴ (Iranian Fisheries Science Research Institute, Agriculture Research Education and Organization (AREEO), Tehran, Iran)
Mahyar Yousefi Siahkalroodi ⁵ (Faculty of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran)

Received: 2024-02-03 Accepted : 2024-07-03 Published : 2024-02-08

Keywords: Antibody, Streptococcosis, Lactococcosis, Vaccine efficiency, Rainbow trout,

Abstract :

For this purpose, 400 to 500 fish with an average weight of 10 gr . Treatment with 10 grams of weight immunized with the trivalent vaccine of Garovak by one-step bath method, treatment of 10 gr immunized with the bath method of trivalent vaccine of Garovak two-step, treatment with weight of 10 gr immunized With the trivalent vaccine of Garovak by the two-step hyperosmotic bath method, the treatment with 10 gr immunized with the one-step injection method of the trivalent vaccine of Garovak and the unvaccinated control treatment were considered. The results showed that the number of red blood cells, blood hemoglobin level, MCV, MCH, MCHC in the vaccinated treatments by the mentioned methods were not significantly different from the control group. But the percentage of blood hematocrit in the treatments There was a significant difference with the control group. Vaccination of fish increased the number of white blood cells in the vaccinated treatments compared to the control group. In this study, based on the results of all the vaccinated groups, antibodies have been produced for both Streptococcus iniani and Lactococcus garvey species in the fishes vaccinated with a simple two-step bath method and vaccine+hyperosmotic medium compared to the one-step vaccinated fishes. was measured significantly higher and the same titer was recorded as the one-step injection vaccine. In this study, the sampling time for fish vaccinated by the two-step bath method was 21 days after the last vaccination, while the sampling for the one-step injection method was 42 days later.