Plantlet Regeneration through Indirect Organogenesis of Flame Gold Tree (Koelreuteria elegans Laxm.)

Subject Areas : Journal of Ornamental Plants

Rameshwar Groach ¹ , Muzafar Hussain Dar ² , Kartar Chand Badgal ³ , Priyanka Pal ⁴ , Narender Singh ⁵ , Kuldeep Yadav ⁶

1 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)
2 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)
3 - Principal, Govt. Degree College, Hiranagar, Kathua
4 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)
5 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)
6 - Department of Botany, Kurukshetra University, Kurukshetra 136119 (India)

Received: 2015-08-08 Accepted : 2015-08-08 Published : 2014-09-01

Keywords: Auxins, Cytokinins, Koelreuteria elegans, Ornamental tree,

Abstract :

Koelreuteria elegans, popularly known as “Flame Gold” is an ornamental tree. In vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified MS medium. The highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-D from leaf segments. Calli transferred in 1.5 mg/l BAP resulted in efficient shoot regeneration (70%) and development (4.35 shoots). MS half strength medium supplemented with 0.2 mg/l NAA reported 80% rooting after 21 days of implantation. Mostly, the roots were long and healthy. Plants were successfully transferred in sterilized mixture of vermiculite: soil: sand (3:1:1) with 65% survival rate under field conditions. The in vitro regenerated plantlets were hardened and acclimatized successfully.

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