تجزیه و تحلیل مقایسه ای برچسب های توالی بیان شده از Picea abies برای شناسایی ژن های مرتبط با تنظیم خواب در مریستم رأس ساقه
Subject Areas : Journal of Ornamental Plants
1 - گروه اصلاح نباتات، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه اصلاح نباتات، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
Keywords: متابولیت‌های ثانویه, تجزیه و تحلیل مقایسه‌ای, رهاسازی خواب, کاتالوگ‌های عملکردی, اختصاصیت ROS,
Abstract :
اساس مولکولی رهاسازی خواب مریستم گیاه یک فرآیند پیچیده است و به خوبی درک نشده است. برای یافتن ژن‌های مربوط به رهاسازی خواب مریستم آپیکال P. abies، از تجزیه و تحلیل برچسب توالی بیان شده استفاده شد. داده های اولیه برای دو کتابخانه cDNA با استفاده از پایگاه داده دانشگاه هاروارد (کتابخانه های خواب و رهاسازی خواب به ترتیب با 6987 و 6981 EST) جمع آوری شد. نرم افزار EGassembler برای جمع آوری تمام توالی های EST به منظور یافتن شباهت بین دو کتابخانه استفاده شد. پس از آن، همه کانتبگ ها با استفاده از نرم افزار CLC bio X-blast در برابر یک پایگاه داده پروتئین غیر تکراری پردازش شدند. برای شناسایی ژن‌های دارای بیان افتراقی در دو کتابخانه، از نرم‌افزار IDEG6 و آزمون Audic-Claverie استفاده شد. ابزار طبقه بندی مقایسه ای GoMapMan برای دسته بندی کاتالوگ های کاربردی استفاده شد. همه یونیژن ها در 35 کاتالوگ عملکردی گروه بندی شدند که 10 کاتالوگ عملکردی متفاوت از جمله متابولیسم اصلی CHO، متابولیسم هورمون، استرس، انتقال، متابولیسم ثانویه، کوفاکتورها و ویتامین ها، متابولیسم نوکلئوتید، تنظیم ردوکس، انتقال الکترون میتوکندری/ATP و تخمیر شناسایی شدند. تاکنون گزارشی مبنی بر نقش متابولیت های ثانویه در تنظیم خواب مریستم گیاهی گزارش نشده است. این مطالعه بینشی در مورد عملکرد احتمالی متابولیت های ثانویه به عنوان تنظیم کننده اصلی خواب مریستم آپیکال در P. abies ارائه می دهد. علاوه بر این، تغییرات ردوکس و اپی ژنتیک در پایین دست هورمون ها نیز به نظر می رسد که در تنظیم خواب دخیل هستند. پتانسیل اختصاصیت ROS از نظر ویژگی های مکانی-زمانی که بیان آنزیم های آنتی اکسیدانی را مشخص می کند به آنها اجازه می دهد تا به عنوان نشانگرهای زیستی در مراحل رشد استفاده شوند. این تحقیق همچنین اطلاعاتی در مورد مکانیسم‌های مولکولی فرآیند مورفوژنز در صنوبر نروژ ارائه می‌کند.
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