واکنش های ریخت شناسی و زیست شیمیایی گل بریدنی ژربرا (Gerbera Jamesonii L.) به کاربرد نانو ذرات سیلیس و کلات کلسیم در شرایط کشت بدون خاک
Subject Areas : Journal of Ornamental Plants
طاهره توفیقی علیخانی
1
(گروه علوم باغبانی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، ایران)
سید جلال طبابایی
2
(گروه علوم باغبانی و مرکز تحقیقات گیاهان دارویی، دانشگاه شاهد، تهران، ایران)
علی محمدی ترکاشوند
3
(گروه خاکشناسی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، ایران)
احمد خلیقی
4
(گروه علوم باغبانی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، ایران)
داریوش طالعی
5
(مرکز تحقیقات گیاهان دارویی، دانشگاه شاهد، تهران، ایران)
Keywords: نشت یونی, شاخص کلروفیل, آنزیم فنیل آلانین آمونیالیاز, خمیدگی ساقه,
Abstract :
هدف از این مطالعه، بررسی واکنش های فیزیولوژیکی گل ژربرا (Gerbera jamesonii L.)تحت تیمار نانو ذرات سیلیس و کلات کلسیم در محلول غذایی بود. برای این منظور، آزمایشی بهصورت فاکتوریل در قالب طرح کاملا تصادفی با دو فاکتور و چهار تکرار انجام شد. فاکتور اول ذرات نانو سیلس (SiO2-Nano) با چهار سطح صفر، ۲۰، 40 و 80 mg/L) از منبعSiO2 و فاکتور دوم کلات کلسیم (Ca-Ch) با چهار سطح صفر، ۶۰، 120 و 240 mg/L) از منبع Ca-EDTA به صورت کاربرد در محلول غذائی بود. قطر گل، طول دمگل، سطح برگ، وزن تر و خشک ریشه، برگ و گل، خمیدگی ساقه، نشت یونی برگ، شاخص کلروفیل، حداکثر کارایی فتوسیستم II (FV/Fm)، درصد سلولز، همی سلولز و هولو سلولز ساقه و فعالیت آنزیم فنیل آلانین آمونیالیاز برگ اندازهگیری شد. نتایج نشان داد اثر متقابل 240 mg/L Ca-Ch همراه با 80 mg/L SiO2-Nano موجب کاهش 5/1 برابری خمیدگی ساقه و کاهش ۲۸ ٪ نشت یونی برگ نسبت به شاهد شدند. تیمار ترکیبی SiO2-Nano 20 mg/L همراه با 60 mg/L Ca-Ch، سطح برگ را نسبت به شاهد ۵۹ ٪ افزایش دادند. بیشترین میزان فعالیت آنزیم فنیل آلانین آمونیالیاز در برگ در60 mg/L Ca-Ch بود (2.91 unit/mg fresh leaves). مناسبترین غلظت بر شاخص کلروفیل، سطح برگ، درصد سلولز و حداکثر کارایی فتوسیستم II (FV/Fm)، تیمار ترکیبی mg/L Ca-Ch 120 همراه با80 mg/L SiO2-Nano بود. بنابراین، کاربرد تیمار ترکیبی 60 mg/LCa-Ch همراه با 40 mg/L SiO2-Nano در محلول غذائی کشت هیدروپونیک گل ژربرا قابل توصیه میباشد.
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