Enhanced Xanthan Gum Production Using Molasses as a Carbon Source in Xanthomonas campestris Fermentation
Subject Areas : Plant Microbiology
Ali Asadi
1
,
Mohammad kazem Momeni
2
1 - Department of Petroleum Engineering, Technical and Engineering Faculty, Islamic Azad University, Tehran, Iran
2 - Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran
Keywords: Xanthan gum production, Xanthomonas campestris, Molasses fermentation, Growth media optimization,
Abstract :
Background & Objectives: Xanthan gum, a microbial polysaccharide produced by Xanthomonas campestris, is widely used in various industries due to its unique rheological properties. The choice of growth medium plays a crucial role in maximizing xanthan production. The aim of this study is to assess the effect of different growth media on xanthan production and to explore molasses as a cost-effective and efficient substrate for Xanthomonas campestris strains.
Materials & Methods: In this study, we evaluated the production of xanthan by two X. campestris strains using two different media: Nutrient Broth and molasses. Xanthan gum was precipitated using isopropanol and calcium chloride, followed by centrifugation to measure yield.
Results: The results showed significant differences in production between the two media. Strain 1 produced 8.00 g/L xanthan in NB medium, while in molasses yielded 45.40 g/L. Similarly, strain 2 produced 9.60 g/L xanthan in NB medium, whereas this strain exhibited the highest yield of 52.00 g/L in molasses. These findings indicate that molasses, due to its rich carbon content, substantially enhances xanthan production compared to NB medium.
Conclusion: This study underscores the potential of molasses as a cost-effective substrate for xanthan production in industrial-scale, especially with high-yielding bacterial strains. Further optimization of fermentation parameters could lead to higher production efficiencies.
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