Phenotypic and genotypic characterization of Pseudomonas savastanoi isolated from winter jasmine, using BOX-PCR, in Shiraz
Subject Areas : Plant MicrobiologyGilda Najafi Pour 1 , Solmaz Hassani 2 , Seyed Mohsen Taghavi 3
1 - Assistant Professor, Department of Plant Protection, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
2 - MS.c., Department of Plant Pathology, Faculty of Agriculture, Shiraz University, Shiraz, Iran.
3 - Professor, Department of Plant Pathology, Faculty of Agriculture, Shiraz University, Shiraz, Iran.
Keywords: Pseudomonas savastanoi, Winter Jasmine, BOX-PCR,
Abstract :
Background and Objectives: Winter jasmine, Jasminum nudiflorum, is a tree belonging to Syringa family, which is used in the urban designing. The gall disease in the tree branches caused by Pseudomonas savastanoi is one of the most important diseases in this trees The purpose of this study was to determine the phenotypic and genotypic diversity of P. savastanoi using the BOX PCR. Materials and Methods: This experimental study was performed on 23 P. savastanoi isolated from winter Jasmine, Shiraz, Fars, Iran. A IAAL primer and PCR approach was used to direct tracking of the strains. The genotypic characteristics of the P. savastanoi isolates was performed by rep-PCR using BOX primers. The data were next analysed using NTsys-PC software. Results: Base in numerical analysis of phenotypic characteristics, the strains showed 88% similarity to each other. Using IAAL primer and GES buffer obtained from gall extract, DNA fragments with 454 bp length were amplified. Using rep-PCR option and BOX primer in NTsys-pc software, it was shown that the isolates can be divided into three cluster with 81% similarity. These clusters belonged to Shiraz’s Winter Jasmine isolates, Tehran’s Oleander isolate and Tehran’s Olive as well as standard isolate. Conclusion: On the basis of our findings, using BOX-PCR it is possible to differentiate the P. savastanoi isolated from different geographical areas and different hosts. Furthermore, this is the first report of direct detection of P. savastanoi from gall using PCR in Iran.