Evaluation the rate of Helicobacter hepaticus in Mus musculus in Esfahan province by PCR

Subject Areas : Molecular Microbiology

Abbas Doosti ¹ , Ali Zohur ² , Maryam Baghernejad ³ , Sadegh Ghorbani-Dalini ⁴

1 - Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
2 - Department of Biology, Payame Noor University, Golpayegan Branch, Golpayegan, Iran
3 - Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
4 - Islamic Azad University, Jahrom Branch, Jahrom, Iran, Young Researcher's Club

Received: 2013-06-15 Accepted : 2013-07-16 Published : 2009-07-01

Keywords: H. hepaticus, 16srRNA, Mus musculus,

Abstract :

Background and Objective: Various Helicobacter species causes contamination of birds , human and other mammals . H.hepaticus infection associated with intestinal , biliary and hepatic disorders such as liver , gall bladder and pancreas cancers . Mus musculus is the most common host of this bacteria that play an important role in transmission of zoonotic infection . The aim of this study was determination of the rate of H.hepaticus in Mus musculuses of Esfahan province by PCR . Material and methods: This was a cross-sectional study, which was done on 261 Mus musculuses isolated in Esfahan in 2009 . Following the description of  mice in steril condition hepatic samples isolated . Then for identification Helicobacter genus and H.hepaticus  , general and  specific primers were used respectively . Results: Helicobacter genus identified in 72% of tatal samples. Out of all Helicobacter genus 42% was be H.hepaticus . Conclusion: According high rate of Helicobacter species infection , it sounds that the  widespread surveillance of   Mus musculuses in studied area was be necessary.