Full-length genome sequencing of an isolate of tomato yellow leaf curl virus to determine its taxonomical place

Subject Areas : Virology

کاووس ایازپور ¹ , Negahdar Oladhossein ² , Ali Pakniat ³

1 - هیات علمی، دانشگاه آزاد اسلامی، واحد جهرم
2 - Department of Plant Pathology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
3 - Plant Protection Department, Fars Research Center for Agriculture and Natural Resources Shiraz, Iran

Received: 2021-01-20 Accepted : 2021-07-27 Published : 2021-08-23

Keywords: Bushehr Province, Begomovirus, Farsc/PAL1v1987 primer, Farsv/TYLCV-[Ab]1997c primer,

Abstract :

Background & Objectives: Tomato yellow leaf curl disease (TYLCD) is one of the most common and important tomato diseases in Iran. Sequences of viral genomes help to find and interbreed    resistant plant varieties. This study aimed to sequence the complete genome of an isolate of the TYLCV, determine its strain and taxonomic status. Materials & Methods: For this purpose, a viral isolate from Assaluyeh was selected and studied. Using degenerate primers of begomoviruses (BC/PCRV 181) a fragment (500bp) of the genome was amplified in a polymerase chain reaction (PCR). According to the sequence of this fragment (500bp), a primer pair was designed (FarsC/FarsV) to achieve the full genome sequence of the virus. By utilizing a combination of primers (FarsC/PAL1V 1978) / (FarsV/TYLCV-[Ab] 1997C) complete sequence of Asalooyeh isolate was amplified and the taxonomic position of that isolate was determined. Results: Phylogenetic analysis showed that this isolate had a one-part genome and was more than 93% similar to the Omani strain. Therefore, this isolate is an Omani variant of the virus. In other researches, was stated that there is only TYLCV-IL strain in Bushehr province, while the results of this study prove the existence of TYLCV-OMN strain. Conclusion: According to the results of this study Asalooyeh isolate of TYLCV belongs to the Omani strain of TYLCV that produces severe yellowing in host plants.