Examination of nucleotide and amino acid sequences of enhancin enzyme in baculoviruses‌

Subject Areas : Virology

Maryam  Rashki ¹ (Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.)
Mojtaba  Mortazavi ² (Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran)

Received: 2023-06-03 Accepted : 2023-10-07 Published : 2023-11-01

Keywords: Baculovirus, codon, enhancin, peritrophic membrane, software,

Abstract :

Background & Objectives: A number of gene groups are conserved in some entomopathogenic baculoviruses, and one of these groups is the enhancin. In the present research, the nucleotide and protein sequence of enhancin and the phylogenetic relationships between them were investigated along with codon analysis of nucleotide sequences and motifs using computer databases. Materials & Methods: Sixty-seven nucleotide and amino acid sequences related to enhancin gene were extracted from GenBank and used to draw a phylogenetic tree based on the maximum likelihood method. Nucleotide sequences related to nine selected genes were selected and extracted from the Sequence Manipulation Suite database to check the frequency of codons. MOTIF Search site was used to find motifs in amino acid sequences. Results: The tree drawn based on nucleotide and amino acid sequences showed two and three main groups, respectively. The sequences of Agrotis segetum granulovirus, Operophtera brumata nucleopolyhedrovirus, and Choristoneura fumiferana multiple nucleopolyhedrovirus were each in their own separate group. In all nine selected nucleotide sequences, the most abundant codons included ATG and TGG that were associated with methionine and tryptophan, respectively. In the amino acid sequences, the conserved sequence HEXXH was identified. Unconserved sequences corresponding to HAISF, HCMAE, QTLGD, HQXXH and HVXXH were found in some sequences. Conclusion: Since the production and secretion of enhancin enzyme as much as possible can be used to increase the insecticidal activity of baculoviruses and be used commercially for pest control, bioinformatics studies to predict the nucleotide and amino acid characteristics of the mentioned proteins in this field, especially with the production of recombinant baculoviruses, can be helpful.

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