Evaluation of DNA break as a prognosis biomarker in Covid-19 patients in Velayat Hospital, Damghan by commet assay

Subject Areas : General Microbiology

elaheh abiri ^{1 *} , Mehdi Mirzaiie ² , majid moghbeli ³ , Amir Atashi ⁴ , Ahad ali harati ⁵

1 - Department of Biology, Damghan Branch, Damghan Islamic Azad University
2 - Medical Sciences, Shahrood University of Medical Sciences
3 - department of biology Damghan branch Islamic Azad university Damghan Iran
4 - Faculty of Medical Sciences, Shahrood University of Medical Sciences
5 - Faculty of Pharmaceutical Sciences, Damghan Islamic Azad University

Received: 2022-02-09 Accepted : 2022-08-09 Published : 2022-09-06

Keywords: Comet assay, COVID-19, DNA Break, ICU,

Abstract :

Background and Objectives: The coronavirus-19 pandemic is a major global health crisis.        Covid-19 is a complex disease with diverse clinical features, from asymptomatic infection to acute respiratory distress syndrome (ARDS) and dysfunction of a number of organs. Prognostic          biomarkers are needed to quickly detect the severity of Covid-19, early in the course of the        disease. The purpose of this study is to explain the amount of DNA break as a biomarker for       disease prognosis in patients with covid-19.Materials and Methods: The comet assay was used to compare the damage caused in the DNA of mononuclear lymphocytes in patients with covid-19 in 4 groups; control, hospitalized in the       normal ward, hospitalized in the ICU and not intubated, hospitalized in the ICU and intubated.Results: The results of examining DNA damage in lymphocytes showed that covid-19 leads to cell damage and especially long-term intubation of covid-19 patients in the ICU, caused more DNA damage and caused more tails increase.Conclusion: The comparison of tails in 4 groups shows that: in people hospitalized in the normal ward, cell damage was less, which could be due to the activation of DNA damage repair systems. As a result, DNA break of lymphocytes has a significant relationship with the prognosis of the disease in patients with Covid-19.  In this study, the comet assay was used to compare the damage caused in the DNA of mononuclear lymphocytes in patients with covid-19 in 4 groups; control, hospitalized in the normal ward, hospitalized in the ICU and not intubated, hospitalized in the ICU and intubated. 

References:

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