Genotypic of Clostridium perfringens in cold area of Kerman province

Subject Areas : General Microbiology

Babak Kheirkhah ¹ , Maryam Hatam Jahromi ²

1 - Assistant Professor, Department of Microbiology, Kerman branch, Islamic Azad University, Kerman, Iran.
2 - MS.c., Department of Microbiology, Sirjan Science and Research branch, Islamic Azad University, Sirja, Iran.

Received: 2015-11-20 Accepted : 2015-11-28 Published : 2016-08-01

Keywords: Multiplex-PCR, Clostridium perfringens, Enterotoxemia,

Abstract :

Clostridium perfringens is an anaerobic, Gram-positive, toxin-forming bacterium and the etiologic cause of enterotoxaemia in animals. Soil is considered as the main source of spore of this bacteria. This study was aimed to genotyping the C. perfringens isolated from intestines of sheep with enterotoxemia and as well as from the soil samples collected from cold area of Kerman province using Multiplex- PCR. This cross-sectional study was carried out on 50 soil samples and 50 intestinal samples collected from three cities located at Kerman to isolate the C. perfringens isolates. The C. perfringens isolates were identified based on enrichment of the samples and growing up the bacteria in specific media and following performance of confirmation tests using biochemical tests. A PCR reaction were performed to confirm the presence of these strains. Overall 42 Clostridial strains were isolated in this study, among them 27 isolates were collected from intestinal samples. Also, 10 soil samples were contaminated with Clostridia, among them 5 samples belonged to C. perfringens. This study showed that C. perfringens is the most prevalent species in the sick sheep and soil in comparison to other species. Furthermore, Type D of this species was more prevalent than others.

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