Isolating tricalcium phosphate solubilizing bacteria from wheat and oat soil in Marvdasht

Subject Areas : Plant Microbiology

Fatemeh Naseri ¹ (MS.c., Department of Microbiology, Shiraz branch, Islamic Azad University, Shiraz, Iran.)
Nima Bahador ² (Assistant Professor, Department of Microbiology, Shiraz branch, Islamic Azad University, Shiraz, Iran.)
Majid Baser ³ (Assistant Professor, Department of Microbiology, Kazeroun branch, Islamic Azad University, Kazeroun, Iran.)
Mehdi Kargar ⁴ (MS.c. Student, Young Researcher’s and Elite Club, Jahrom branch, Islamic Azad University, Jahrom, Iran.)

Received: 2014-09-02 Accepted : 2014-12-02 Published : 2015-05-01

Keywords: 16S rRNA, Wheat fields, Oat fields, Tricalcium phosphate, Enterobacteriaceae,

Abstract :

Background & Objectives: Many soil microorganisms are ale to solve unavailable phosphorus through metabolic activity and secretion of organic acids. This study was aimed to isolate tricalcium phosphate active solubilizing bacteria from wheat and barley farms in Marvdasht Plain. Materials & Methods: This cross-sectional study was conducted on 50 barley and 50 wheat farms in Marvdasht Plain. The soil samples were enriched in NRIP media containing 10 percent tricalcium phosphate. The tricalcium phosphate solubilizing bacteria which produced halos on media were determined through biochemical tests, PCR and sequencing techniques. The activity of two active bacteria isolated from wheat and barley farms were analysed by HPLC method in order to investigate the production of secreted organic acids. Results: Overall 9 (18%) and 6 (12%) of the tricalcium phosphate solubilizing bacteria were isolated from the wheat and barley farms located at Marvdasht, respectively. The HPLC analysis showed that the microorganisms isolated from barely and wheats farms produce 4 and 3 respectively, different low molecular weight acids. Conclusion: Since most of the tricalcium phosphate active solubilizing bacteria were isolated from the barely farms, these bacteria can be found in the soils in which less Phosphorus fertilizers were used. Screening of these bacteria in different farms and their identification can be useful in the production of biologic fertilizers and growth of plants in that area.