Biodegradation of formaldehyde by isolated bacteria from wastewater of paint production lant Pars Negin Nama in Mazandaran province
Subject Areas : Environmental Microbiology
Safa
Hashemi Jirdehi
1
(M.Sc., Department of Microbiology, Tonekabon branch, Islamic Azad University, Tonekabon, Iran.)
Saba
Amiri Kojuri
2
(M.Sc., Young Researchers and Elite club, Chalus branch, Islamic Azad University, Chalus, Iran.)
Zoheir
Heshmatipur
3
(Assistant Professor, Department of Microbiology, Tonekabon branch, Islamic Azad University, Tonekabon, Iran.)
Keywords: Spectrophotometer, Formaldehyde, Biological degradation,
Abstract :
Background & Objectives: Formaldehyde is a toxic and hazardous compound and s known as one of the mutagenic and carcinogenic agents in living creatures. Among a variety of procedures designed for the elimination of contamination, biological removal is capable of converting pollutants to innocuous and nontoxic substances using less amount of energy and chemicals. This study was aimed biodegradation of formaldehyde by the bacteria isolated from Wastewater of Paint Production (Pars Negin Nama). Materials & Methods: In this analytical study, samples were taken from paint production factory effluent. The samples were added to CM and MSM media to isolate bacteria. The bacteria were analyze based on morphological and biochemical characteristics. The bioregdaration ability of bacteria in MSM containing formaldehyde media was evaluated in absorbance at 600 nm using a spectrophotometer. The identity of bacteria was determined based on sequencing of 16SrRNA. Results: The result showed that the best formaldehyde degrading bacteria belonged to a new strain referred to as Bacillus sp. Safa. Toneka. This strain was able to degrade formaldehyde 1500 ppm formaldehyde in MSM agar and 2000 ppm formaldehyde in MSM broth after 48 hour. Conclusion: The results of the present research showed the ability of bacteria in the terms of the elimination of formaldehyde in contaminated areas.