Isolation of chitinase-producing archaea from shrimp shells in Hormuz Island of Iran

Subject Areas : Environmental Microbiology

Farhanaz Sadat Mirlohi ¹ , soheila Abbasi ² , maryam Jalilitabaii ³ , Giti Emtiazi ⁴

1 - Student of MSc, Department of Microbiology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran
2 - Assistant professor, Department of Microbiology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran.
3 - Assistant professor, Department of Microbiology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran.
4 - Professor, Department of Microbiology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran.

Received: 2024-05-29 Accepted : 2024-08-31 Published : 2024-09-10

Keywords: chitinase, archaea, colloidal chitin, Hormuz Island.,

Abstract :

Background and Objectives: Chitin is the second most abundant polymer on the earth, that found in the cell wall of fungi, the exoskeleton of insects and crustaceans. Chitin is decomposed by chitinases and the compounds derived from them can be very useful and beneficial for treating some diseases. The production of this enzyme by halophilic microorganisms, especially archaea, increases the value of these enzymes in industries due to their ability to withstand harsh conditions.

Materials and methods: The isolates of this study were obtained by sampling from several areas of Hormuz Island in the Persian Gulf of Iran and cultivating them in DSM medium with shrimp shell as the only carbon source. Quantitative measurements were performed to examine the amount of chitinase enzyme in the isolates and select the best isolate, followed by the necessary tests to identify the selected isolate.

Results: The outcomes indicated the presence of chitinase-producing archaea with different amounts of the enzyme based on the consumption of colloidal chitin as the sole carbon source. The selected isolate (E) (Halarchaeum rubridurum ASFM1402) had a high potential (9.12 IU/mL) produce large amounts of chitinase enzyme from colloidal chitin.

Conclusion: Considering the geographical conditions of the southern regions of the country and the presence of the sea and the fishing industry, as well as the abundant chitinous wastes resulting from them, it is important to find ways to remove these wastes in such a way that they do not have harmful environmental effects. Our research can be the beginning of extensive research in this area.

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