Frequency of methicillin resistant (mecA) and panton-valentine leucocidin (pvl) genes among Staphylococcus aureus isolates recovered from clinical samples of Rasht hospitals

Subject Areas : Medical Microbiology

Maryam Rahimpour Hesari ¹ , Amir Mirzaie ² , Ali Salehzadeh ³

1 - MS.c., Department of Biology, Rasht branch, Islamic Azad University, Rasht, Iran.
2 - BS.c., Young Researchers and Elite Club, East Tehran branch, Islamic Azad University, Tehran, Iran.
3 - Assistant Professor, Department of Biology, Rasht branch, Islamic Azad University, Rasht, Iran.

Received: 2015-10-25 Accepted : 2016-01-04 Published : 2016-06-01

Keywords: Staphylococcus aureus, mecA gene, pvl gene,

Abstract :

Background & Objectives: Staphylococcus aureus is one of the most important nosocomial infection agent and now methicillin-resistant S. aureus have spread. Also, panton-valentin leukocidin is one of important virulence factors in S. aureus. The study was aimed to evaluate the antibiotic resistant, frequency methicillin resistant and panton- valentin leukocidin genes in clinical S. aureus isolates from hospitals of Rasht, Iran. Materials & Methods: This cross-sectional study was carried out on 250 clinical samples collected from hospitals of Rasht during one year (2013-2014). The S. aureus isolates were identified by microbiological methods. Antibiotic sensitivity was performed by CSLI method using disk diffusion method. In addition, the presence of methicillin resistant (mecA) and panton-valentin leukocidin (pvl) genes were evaluated using polymerase chain reaction (PCR). Results: Totally, 50 S. aureus isolates were recovered. The results of antibiotic susceptibility tests showed that 34 out of 50 S. aureus isolates (68%) were resistant to methicillin. In addition, the prevalence of mecA and pvl gene among isolates were 60% (30 isolates) and 20% (10 isolates), respectively. Conclusion: This study showed increased resistance to different antibiotics in S. aureus that is a serious warning to the treatment of infections caused by this bacterium in the region.

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