Determination of antibiotic susceptibility pattern of Pseudomonas aeruginosa and the prevalence of bla OXA-23 gene in isolates from a Hospital in North Khorasan

Subject Areas : Medical Microbiology

Mohadeseh Radmehr ¹ , majid moghbeli ²

1 - Ph.D Candidate, Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran.
2 - department of biology Damghan branch Islamic Azad university Damghan Iran

Received: 2021-10-02 Accepted : 2022-02-17 Published : 2021-12-22

Keywords: Antibiotic susceptibility, Pseudomonas aeruginosa, gyrB gene, bla OXA-23 gene,

Abstract :

Background & Objectives: Pseudomonas aeruginosa is one of the most important pathogens of opportunistic infections, which is considered as one of the main causes of nosocomial infections. The aim of this study was to isolate pseudomonas aeruginosa and identify strains with                  bla OXA-23 gene using polymerase chain reaction (PCR) technique. Materials & Methods: In this study, 70 isolates of pseudomonas aeruginosa from various            infections including lung, urine, blood, wound and sputum were collected in north khorasan       hospital, after phenotypic and genotypic confirmation of the isolates, antibiotic discs of doripenem and imipenem (Rosco), meropenem and ciprofloxacin, colistin and amikacin (Mast) were used to perform antibiotic susceptibility testing of bacteria by disk diffusion method. Results: Isolates showed 32.8%, resistance to amikacin , 47.1% todoripenem, 37.1% to imipenem, 40% to ciprofloxacin, and 10% to colistin. The highest resistance of the strains was related to meropenem 61.4% and the lowest was colistin with 10% resistance. Conclusion: In this study, the resistance of pseudomonas aeruginosa to the antibiotic meropenem was 61.4% and compared to other studies, including in ethiopia, showed a higher level of           antibiotic resistance, this level of carbapenem resistance indicates an increase in resistance of pseudomonas aeruginosa strains to carbapenems.