Identification of some heterocystous cyanobacteria isolated from rice fields of Mazandaran province with emphasis on multi-genic approach

Subject Areas : Environmental Microbiology

Sara Kabirnataj ¹ , Ghorban Ali Nematzadeh ² , Ahmad Farhad Talebi ³ , Meisam Tabatabai ⁴ , Prashant Singh ⁵

1 - Ph.D. candidate, Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 - Professor, Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
3 - Assistant Professor, Faculty of Biotechnology, Semnan University, Semnan, Iran
4 - Assistant Professor, Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran
5 - Assistant Professor, Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, India

Received: 2019-07-30 Accepted : 2019-08-28 Published : 2019-11-22

Keywords: Taxonomy, Cyanobacteria, Heterocyst, multi-genic, rpoC1 gene,

Abstract :

Background & Objectives: In the past, the classification of cyanobacteria was only based on the morphological characteristics; however other markers such as accurate molecular methods have been recently used. This study aimed to identify several heterocystous cyanobacteria by polyphasic taxonomy and to investigate the multigenic approach in enhancing the accuracy of  cyanobacterial identification. Materials & Methods: After analyzing the morphological features and initial identification, the 16SrRNA gene along with tufA, rbcL, psbA, and rpoC1 functional genes were used to evaluate the taxonomic status and phylogenetic relationships. For this purpose, after DNA extraction, amplification and sequencing of gene fragments, the exact position of the strains was determined by making phylogenetic trees, based on the corresponding genes using MEGA software. Results: Among  the functional genes studied, the rpoC1 gene was able to discriminate genera very well, a result that completely confirmed 16SrRNA phylogenetic results. Finally based on the results, 4 samples from the Desmonostoc genus and 2 samples from the Calothrix genus were introduced.  Conclusion: The results of this study demonstrate the ability of the rpoC1 gene to discriminate genera correctly and confirm the morphological and phylogenetic results of 16SrRNA gene analysis. A phylogenetic study using the rpoC1 gene marker helps to clarify phylogenetic relationships among cyanobacteria. Moreover, it provides further evidence of their chloroplast origin and the presence of different evolutionary pathways among them.

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