Evaluation of the Antifungal Effects of Vacciniumarctostaphylos (Blackberry) Fruit Extract on Candida albicans and Candida glabrata under Laboratory Conditions
Subject Areas : Experimental Studies
ahmad halakou
1
,
Maryam Kazemi
2
,
مریم رفیعی راد
3
*
,
Mottahareh Eskandari
4
1 - faculty member Islamic Azad University izeh branch
2 - - Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran
3 -
4 - IAU,,.shahrekord Branch
Keywords: Candidiasis, Candida albicans, Candida glabrata, Blackberry extract,
Abstract :
Introduction:Candidiasis encompasses a spectrum of diseases, ranging from superficial and mucosal infections to severe and life-threatening systemic infections. Candida glabrata is the fourth most common cause of candidiasis after Candida albicans. The overuse of synthetic antifungal drugs has led to the emergence of resistant strains. On the other hand, medicinal plants, rich in antioxidants and secondary metabolites, are considered safe and cost-effective alternatives to chemical drugs. Objective: This study aimed to investigate the antifungal effects of Vaccinium arctostaphylos (blackberry) fruit extract on Candida albicans and Candida glabrata. Methods: The blackberry fruits were dried, dissolved in 85% methanol, and filtered to obtain the extract. The antifungal activity of the extract was evaluated using the microdilution method at concentrations ranging from 2 to 500 µg/mL against standard strains of Candida albicans and Candida glabrata in microplate wells. Results: The results demonstrated that the extract inhibited the growth of both Candida species. The minimum inhibitory concentration (MIC) of the extract for Candida albicans and Candida glabrata was 32 and 16 µg/mL, respectively. Additionally, the minimum fungicidal concentration (MFC) for Candida albicans and Candida glabrata was 125 and 64 µg/mL, respectively. Conclusion: Given the significant inhibitory effects of Vaccinium arctostaphylos extract on both Candida albicans and Candida glabrata, this extract can be introduced as an effective antifungal agent under laboratory conditions. Further clinical studies are recommended to evaluate its antifungal potential.
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