Study of Antibacterial and Synergistic Effect of Digitalis nervusa Extract and Ciprofloxacin on Staphylococcus aureus

Subject Areas : Food Microbial Contamination

leili  Ayoubinejad ¹ (M.Sc Student of Food Science and Technology, Qhuchan Branch, Islamic Azad University, Qhuchan, Iran.)
hamid  nazari ² (M.Sc Student of Food Science and Technology, Qhuchan Branch, Islamic Azad University, Qhuchan, Iran.)
Ali  Mohammadi sani ³ (Department of Food Science and Technology, Qhuchan Branch, Islamic Azad University, Qhuchan, Iran)

Received: 2015-10-20 Accepted : 2016-04-26 Published : 2016-05-01

Keywords: Ciprofloxacin, Antibacterial, Synergistic effect, Ethanol extract, Digitalis,

Abstract :

In spite of novel progresses in the methods for foodstuff’s preparation and production, consumer’s health and safety in the public hygiene import increasingly. One of the ways to control sickening microorganisms is the use of chemical retentive in the food. Today consumers are complaining to use natural retentive that are derivatives of herbaceous resources. As for harmful effects of food’s synthetic and chemical retentive. This study performs as for determining antibacterial Effects of Digitalis plant’s extracts on Staphylococcus aureus pathogenic bacteria in the foodstuff. In this study the ethanol extract alone and in combination with ciprofloxacin antibiotic on gram-positive bacteria, Staphylococcus aureus (ATCC25923), were evaluated by micro broth dilution method. MIC test results for Digitalis plant extract and Ciprofloxacin antibiotic evaluated 4.9 mg/ml and 0.5 μg/ml. effect of both extract and antibiotic results showed that growth of Staphylococcus aureus in the simultaneous use of 3.5 mg/ml extract and 0.28 μg/ml was inhibited by antibiotics. Evaluation of MBC tests for Digitalis plant extract and ciprofloxacin antibiotic evaluated 7.1 mg/ml and 1 μg/ml, results show that the combination of 4.2 mg/ml of extract and 0.14 μg/ml antibiotics cause the bacteria is eliminated.Concomitant use of alcohol extract and antibiotics, decreasing 48 percent of antibiotic to inhibit the growth of Staphylococcus aureus, and simultaneous use of alcohol extract and antibiotic, antibiotic use decreased 86 percent in the MBC for Staphylococcus aureus.