Anti-adhesively effect of bio-surfactant from Lactobacillus rhamnosus ATCC 7469 on the isolated bacteria from the pasteurized milk production line

Subject Areas : Food Microbial Contamination

Sayedeh Saleheh Vaezi ¹ , Arezoo Tahmourespoor ²

1 - Young Researchers and Elite Club, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 - Department of Basic Medical Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Received: 2016-04-24 Accepted : 2016-10-19 Published : 2016-10-22

Keywords: Bacteria, biofilm, Bio-surfactant, Anti-adhesively, Optical density,

Abstract :

Many bacteria possess the ability to connect to surfaces that this ability to "biofilm" is called. Biofilms attached to the food industry for the production of many problems in the production line and the final product is therefore necessary to examine methods of controlling them. In this study, the bacterial biofilm from the inner part of pasteurized milk production line from one factory in Isfahan were isolated, purified and identified (by biochemical tests). After that, bio-surfactant was extracted from Lactobacillus rhamnosus ATCC 7469. Then the anti-adhesively effect of bio-surfactant was performed by micro-titer-plate method and optical density was read with ELISA Reader. Finally, data factorial analysis of variance to test and comparison of means were analyzed method LSD at 5%. Results showed Staphylococcus was the most predominant isolated genus with 19% of the whole population. Also the average of anti-adhesively effect of bio-surfactants used in this study is 42.8%. The highest effect of bio-surfactants was on Klebsiella pneumonia with 82.3% for reducing connection and the least effect was on Staphylococcus aureus with 4.2%forreducing connection. However, due to the positive anti-adhesively effect of bio-surfactant, use it in the production line during the CIP process is recommended.