Simultaneous spectrophotometric determination of lidocaine and hydrocortisone acetate in pharmaceutical preparations by multivariate calibration methods

Subject Areas : Journal of the Iranian Chemical Research

Amir H.M. Sarrafi ¹ , Manochehr Bahmaei ² , Assieh Z. Mousavi ³

1 - Department of Chemistry, Faculty of Science, Islamic Azad University-Central Tehran Branch, Tehran, Iran
2 - Department of Chemistry, Faculty of Chemistry, Islamic Azad University-North Tehran Branch, Tehran, Iran.
3 - Department of Chemistry, Faculty of Chemistry, Islamic Azad University-North Tehran Branch, Tehran, Iran.

Received: 2009-09-27 Accepted : 2009-11-03 Published : 2010-12-31

Keywords: Spectrophotometric, Lidocaine, Hydrocortisone acetate, Multivariate calibration,

Abstract :

The multivariate calibration method, partial least square regression (PLS) was applied for thesimultaneous spectrophotometric determination of Lidocaine (LID) and Hydrocortisone acetate(HCA) in their mixtures. The parameters of chemometric technique were optimized and theproposed method was validated with synthetic samples and applied to analyze these drugs inpharmaceutical products with good accuracy and precision. The results were compared with theHPLC method. The squares of correlation coefficients (R2) for predicted LID and HCA with theproposed method in test samples were 0.9970 and 0.9964, respectively. The relative standarddeviations for commercial products were less than 1%.

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