Micropropagation of Clematis orientalis L. culture in vitro
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
علی
ایزدی صادق آبادی
1
(گروه علوم باغبانی، ، دانشکده کشاورزی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، ایران)
احمد
خلیقی
2
(گروه علوم باغبانی، ، دانشکده کشاورزی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، ایران)
عبدالله
قاسمی پیربلوطی
3
(گروه گیاهان دارویی، دانشکده کشاورزی، دانشگاه آزاد اسلامی، واحد شهرکرد، ایران)
مرضیه
تقی پوردهکردی
4
(گروه زیست شناسی گیاهی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات فارس،)
Keywords: Micropropagation, benzyl amino purine, Clematis orientalis, Naphthalene acetic acid (NAA),
Abstract :
Background & Aim: Clematis is an ornamental and medicinal plant that is primarily planted as anornamentalplant. Clematis orientalis is native to Asia and Central Europe, used as an antiseptic and refrigerant. The propagation of Clematis is very important. In this study, the effects of plant growth regulators on rooting and growth of the plants in vitro culture were examined. Experimental: In this study, effects of growth regulators, including naphthalene acetic acid (NAA), at the concentrations of 0.0, 0.4, 0.5, and 0.6 mg/mL and Indol butyric acid (IBA) at the concentrations of 0.0, 0.4, 0.5, and 0.6 mg/mL on micropropagationin Murashige and Skoog’s medium (MS) were investigated. Results & Discussion: Results indicated that the effects growth regulators of auxin at different concentrations on in vitro growth micrpropagules were significant. The highest rooting and number of stems were obtained from control. Increase concentration of growth regulators decreased rooting. The highest decrease rate was obtained from0.6 mg/L auxin. Recommended applications/industries: The result of study indicated that positive effects lateral shoot explants and Murashige and Skoog’s medium (MS) without growth regulators on micropropagation.
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