Application of HPTLC in the Assessment of the Extraction Process of Stevia Rebaudiana Using Ultrasound Waves

Subject Areas : Microbiology

M. Rouhani ¹ , Z. Mirjafari ² , H. Rezapour ³ , J. Mokhtari ⁴

1 - Assistant Professor of the Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Assistant Professor of the Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - MSc Student of the Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.
4 - Assistant Professor of the Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Received: 2019-12-26 Accepted : 2019-12-26 Published : 2019-12-22

Keywords: HPTLC, Stevioside, Stevia rebaudiana, Ultrasound,

Abstract :

Introduction: In the recent decades, the increase in population, decrease in physical activities and consumption of improper food to fulfill the nutritional need have caused various diseases namely diabetes. The extract from the leaves of Stevia Rebaudiana plant might be employed as a natural sugar substitute. The aim of the present study is to employ ultrasound waves to optimize the extraction process from the leaves of Stevia Rebaudiana and use High Performance Thin Layer Chromatography (HPTLC) to analyze the extract. Materials and Methods: Water, water-methanol mixture, methanol, water-ethanol mixture and ethanol was applied for extraction as solvent. The extraction was carried out by soxhlet and ultrasound waves at various times and power condition according to the official procedures. Results: The results indicated that water was the most suitable solvent among the solvents employed. The results also showed that the application of ultrasound with power of 90 W for 2 minutes resulted better extraction (811.61 g/mlμ) as compared to the soxhlet method (795.70 g/mlμ). Conclusion: The present study indicated that ultrasound application might be a suitable, fast and safe method for extraction of Stevioside sweetener from Stevia Rebaudiana.

References:

Abdullateef, R. A. & Osman, M. (2012). Studies on effects of pruning on vegetative traits in Stevia rebaudiana Bertoni (Compositae)". International Journal of Biology, 4 (1), 26-32.

Attimarad, M., Ahmed, K. K. M. & Harsha, S. (2011). High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery. Pharmaceutical Methods, 2 (2), 71-75.

 

Baştürk, A., Ceylan, M. M., Çavuş, M., Boran, G. & Javidipour, I. (2017). -Ultrasound assisted rapid extraction and kinetic modelling of influential factors: Extraction of camptothecin from Nothapodytes nimmoniana plant. Ultrasonics Sonochemistry, 37 (12), 582-591.

Cardello, H. M. A. B., Da Silva, M.A.P.A. & Damasio, M. H. (1999). "Measurement of the relative sweetness of stevia extract, aspartame and cyclamate/saccharin blend as compared to sucrose at different concentrations". Plant Foods for Human Nutrition. 54 (2), 119–129.

Formigoni, M., Milani, P. G., Avíncola, A. d. S., Santos, V. J. D. & da Costa, S. C. (2018). Effects of some herbal extracts on oxidative stability of corn oil under accelerated oxidation conditions in comparison with some commonly used antioxidants. LWT, 89 (5), 358-364.

Sundlof, M. T. (2018). Has Stevia been approved by FDA to be used as a sweetener? US Food and Drug Administration.

Hemwimol, S., Pavasant, P. & Shotipruk, A. (2006). Ultrasonic-assisted extraction of anthraquinones from roots of Morinda Citrifolia. Ultrasonics Sonochemistry, 13 (6), 543-548.

Jimenez, A. & Beltran, G. (2007). High-power ultrasound in olive paste pretreatment. Effect on process yield and virgin olive oil characteristics. Ultrasonics Sonochemistry, 14(6), 725-731.

Kolb, N., Herrara, J. L., Ferreyra, D. J. & Uliana, R. F. (2001). Analysis of Sweet Diterpene Glycosides from Stevia rebaudiana:  Improved HPLC Method. Journal of Agricultural and Food Chemistry, 49 (10), 4538-4541.

Martino, E., Ramaiola, I. & Urbano, M. (2006). Microwave-assisted extraction of coumarin and related compounds from Melilotus officinalis (L.) Pallas Alternative to Soxhlet and ultrasound-assisted extraction. Journal of Chromatography A., 1125 (2), 147-151.

Martins, P. M., Thotat, B. N., Lanchote, A. D. & Freitas, L. A. (2016). Green extraction of glycosides from Stevia rebaudiana (Bert.) with low solvent consumption: A desirability approach, Resource-Efficient Technologies, 2 (4), 247-253.

Martins, P. M., Lanchote, A. D., Thorat, B. N. & Freitas, L. A. P. (2017). Turbo-extraction of glycosides from Stevia Rebaudiana using a fractional factorial design, Revista Brasileira de Farmacognosia, 27 (4), 510-518.

McClements D. J. (1995). Advances in the application of ultrasound in food analysis and processing. Trends in Food Science & Technology, 6 (9), 293–299.

Misra, H., Soni, M., Silawat, N., Mehta, D., Mehta, B. K. & Jain, D. C. (2011). "Antidiabetic activity of medium-polar extract from the leaves of Stevia rebaudiana Bert. (Bertoni) on alloxan-induced diabetic rats". Journal of Pharmacy and Bioallied Sciences, 3 (2), 242–248. 

Morlock, G. E., Oellig, C., Bezuidenhout, L. W., Brett, M. J. & Schwack, W (2010). "Miniaturized planar chromatography using office peripherals". Analytical Chemistry. 82 (7), 2940–2946. 

Myers, R.H. & Montgomery, D.C. )2002(. Response surface methodology process and product optimization using designed experiments, Wiley Pub Inc, 51-83.

Nurok, D. (1989). "Strategies for optimizing the mobile phase in planar chromatography". Chemical Reviews, 89 (2): 363–375.

Parsons, W. T. & Cuthbertson, E. G. (2001). Noxious Weeds of Australia, 2nd ed. Collingswood, Australia: CSIRO Publishing. 289-294.

Patil, D. M. & Akamanchi K. G. (2015). Ultrasound-assisted extraction of polyphenols from native plants in the Mexican desert. Ultrasonics Sonochemistry, 22 (4), 474-481.

Paz, J. E. W., Márquez, D. B. M. Ávila, G. C. G. & Cristoba R. E. B. (2009), CAMAG Bibliography Service 103, 5-25.

Salazar, V. A. G., Encalada, S. V., Cruz, A. C. & Campo, M. R. S (2018). Pretreatment with ethanol as an alternative to improve steviol glycosides extraction and purification from a new variety of stevia. Food Chemistry, 241 (15), 452-459.

Salazar, V. A. G., Encalada, S. V., Cruz, A. C. & Campos, M. R. S. (2018). Stevia Rebaudiana: A sweetener and potential bioactive ingredient in the development of functional cookies. Journal of Functional Foods, 44, 183-190.

Stones, M. (2011). "Stevia wins final EU approval". Food manufacture.co.uk. Retrieved 22 November 2011.

Vinatoru, M. (2001). An overview of the ultrasonically assisted extraction of bioactive principles from herbs. Ultrasonics Sonochemistry, 8 (3), 303-313.