Ethanol Washing, Pectinase Pretreatment and Oleoresin Saponification of Tomato Waste to Increase Lycopene Purity

Subject Areas : Microbiology

A. Ranjbar Nedamani ¹ (Ph. D. Graduated of the Department of Food Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Golestan, Iran.)
Y. Maghsoudlou ² (Professor of the Department of Food Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Golestan, Iran.)
M. Ghorbani ³ (Associate Professor of the Department of Food Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Golestan, Iran.)
A. R. Sadeghi Mahounak ⁴ (Associate Professor of the Department of Food Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Golestan, Iran.)

Received: 2019-07-02 Accepted : 2019-07-02 Published : 2019-06-22

Keywords: Ethanol Treatment, Lycopene, Oleoresin, Pectinase, Saponification, Tomato Waste,

Abstract :

Introduction: In this study the best method of extracting lycopene from tomato skin, waste and the effect of pectinase enzyme pre-treatment at 2, 4, 6, 8, and 10 ml/Kg concentrations and 30, 60, 90 minutes of effective times for tomato waste and also washing with 94% ethanol at 60 °C for 5 seconds were studied in order to determine the best method of increasing lycopene purity in oleoresin. Materials and Methods: Lycopene extraction was performed at 30 °C for 16 hours with hexane: ethanol: acetone (1:1:2) at ratios of 1:10 to dried samples with gentle stirring. In order to prevent the lycopene oxidation 0.05 percent w/w of BHT was used. The effect of oleoresin saponification to increase the purity of lycopene in oleoresin was also studied by propylene glycol and potassium hydroxide 45% (w/w) and deionized water at the concentration of 30%, 10% and 10% oleoresin by weight, respectively. Results: The effect of enzyme pretreatment and ethanol and also oleoresin saponification had a significant effect on oleoresin and lycopene extraction and extraction efficiency from tomato waste (P<0.01). Ethanol treatment significantly increased lycopene purity in oleoresin (P<0.01). In waste samples treating with 10 ml/kg enzyme concentration at 90 minutes of effective time resulted in highest extraction of lycopene (279.5 mg/100g oleoresin). Conclusion: It has been shown that if saponification is not employed in lycopene extraction from tomato waste the ethanol washing pre-treatments should not be applied.

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