Effect of nickel and pH on antioxidant activity, and total phenolic and flavonoid contents of Cladophora glomerata

Subject Areas : environmental management

Zohreh Senobari ¹ , Naser Jafari ² , Mohammad Ali Ebrahimzadeh ³

1 - Department of Biology, Faculty of Science, University of Mazandaran, Babolsar, Iran
2 - Department of Biology, Faculty of Science, University of Mazandaran, Babolsar, Iran
3 - Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, mazandaran, Iran

Received: 2013-04-26 Accepted : 2014-01-08 Published : 2014-07-23

Keywords: Cladophora glomerata, Total phenolic, Flavonoid, Antioxidant Activity,

Abstract :

Excess Ni concentration is considered as a stressful factor for algae, and can affect their growth as alimiting factor. Ni toxicity can also induce oxidative stress in algae and plants by producing freeradicals. This study aimed to evaluate the effect of different concentrations of Ni and pH onantioxidant activity, total phenolic and flavonoid contents. Total phenolic and flavonoid contents ofextracts were determined by Folin-Ciocaltue assay and expressed based on mg of gallic acid per g ofextract. Then the antioxidant activity of ethanolic extracts was assessed by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH). In this study, the highest phenolic content (1873 mg gallic acid/g extarct) andflavonoid content (1106 mg quercetin/g extarct) were obtained at pH=5. In the antioxidant activitytest, the maximum IC50 was recorded at pH=9. The results indicated that ethanolic extract ofCladophora glomerata has a noticeable antioxidant ability against various oxidative systems and itcan be used as an accessible source of natural antioxidant in food and pharmaceutical industries.

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