Study on lethal Effects on Silver Nano Particles of Goldfish (Carassius Auratus Gibelio)

Subject Areas : Environmental pollutions (water, soil and air)

Mohammad Forouhar Vajargah ¹ , Mohammad Reza Imanpoor ² , Ali Shabani ³ , Aliakbar Hedayati ⁴

1 - Postgraduate of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Iran. *(Corresponding Author)
2 - Full Professor, Department of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Iran.
3 - Associate Professor, Department of Aquaculture, Gorgan University of Agricultural Sciences and Natural Resources, Iran.
4 - Associate Professor, Department of Aquatics Production and Exploitation, Gorgan University of Agricultural Sciences and Natural Resources, Iran.

Received: 2016-06-29 Accepted : 2016-09-07 Published : 2021-07-23

Keywords: nano-toxicology, Aquatic, silver, Heavy Metals,

Abstract :

Background and Objective: Due to the increasing use of nano-materials, especially nano silver because of the anti-bacterial inevitable is used in various industries, it will entry into the ecosystems, so in this study, the toxic response of nano silver on goldfish was studied.Material and Methodology: To determine the toxicity of nano-silver, O.E.C.D standard method number 203 was used. Mortality recorded was daily basis over a period of 96 hours and after records, to determine the lethal toxicity in the 24, 48, 72 and 96 hours, probit software was used. Finally, the maximum allowable concentration and degree of toxicity was found.Findings: During the period of 24 to 96 hours with increasing concentrations of nano silver, mortality of fish was increased. The mortality rate at concentrations of 0, 0.01, 0.1, 0.5, 1, 2.5 and 5 mg after 24 hours were 0,0,0,0, 4, 11 and 21 respectively, in concentrations of 0, 0.01, 0.1, 0.5, 1, 2.5 and 5 mg after 96 hours, mortality rates were 0,0,0, 10, 21, 21 and 21, respectively. Based on these results, the lethal concentration (LC50) of nano silver on goldfish in time 24, 48, 72 and 96 hours were obtained 2.31 and 1.48 and 0.995 and 0.53 ppm respectively.Discussion and Conclusion: In general, the results show that the toxicity of silver nano is very high during the finger period and its side effects should not be ignored with the increasing spread of this substance. It is also necessary to compare the results with silver ions when evaluating the toxic effects of nano-silver. 

References:

1. Hedayati A, Vajargah MF, Yalsuyi AM, Abarghoei S, Hajiahmadyan M. Acute toxicity test of pesticide abamectin on common carp (Cyprinus carpio). Journal of Coastal Life Medicine. 2014;2(11):841-4.


2. Tarbali Neda, Bahavar Maedeh, Ainollahi Nahid, Fariba Planters. The effect of silver nitrate on horseradish peroxidase activity. Feyz Scientific-Research Bi-Monthly. 1391; 16 (7): 713-714


3. Boenigk, Jens. 2014. Effects of Silver Nitrate and Silver Nanoparticles on a Planktonic Community: General Trends after Short-Term Exposure. PloS one: 95-340.‏


4. Vajargah MF, Hedayati A, Yalsuyi AM, Abarghoei S, Gerami MH, Farsani HG. Acute toxicity of Butachlor to Caspian Kutum (Rutilus frisii Kutum Kamensky, 1991). Journal of Environmental Treatment Techniques. 2014;2(4):155-7.


5. Farahbakhsh A, Naeimi A, Movahedi A, Ahrar A, Mozaffari M, Sehati N. 2006, Introduction to Nanotechnology, 503 pages.


6. Khayyam Nekouei. M, Biazar A, Jozaei Gh, 2010, Nanotechnology in Agricultural Sciences, 241 pages.


7. Haredasht M, Mirvaghefi AR. AR, 2012, Applications of Nanotechnology in Fisheries, Nanotechnology Monthly, 11 (6): pp. 15-13.


8. Gong, P., Li, H., He, X., Wang, K., Hu, J., Tan, W., & Yang, X. 2007. Preparation and antibacterial activity of Fe₃O₄-Ag nanoparticles. Nanotechnology, 18(28), 285604.‏


9. Blaise, C., Gagne, F., Ferard, J. F., & Eullaffroy, P. 2008. Ecotoxicity of selected nano‐materials to aquatic organisms. Environmental toxicology, 23(5), 591-598.‏


10. Chen, X., & Schluesener, H. J. 2008. Nanosilver: a nanoproduct in medical application. Toxicology letters, 176(1), 1-12.


11. Vajargah MF, Hedayati A. Acute toxicity of trichlorofon on four viviparous fish: Poecilia latipinna, Poecilia reticulata, Gambusia holbrooki and Xiphophorus helleri (Cyprinodontiformes: Poeciliidae). Journal of Coastal Life Medicine. 2014;2(7):511-4.


12. Banaee M., Sureda A., Mirvaghefi AR., Ahmadi K. 2010. Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss). Pesticide Biochemistry and physiology.


13. Zhang, X. D., Wu, H. Y., Wu, D., Wang, Y. Y., Chang, J. H., Zhai, Z. B.,. & Fan, F. Y. 2009. Toxicologic effects of gold nanoparticles in vivo by different administration routes. International journal of nanomedicine, 5, 771-781.


14. M and Mesbah. M, 2010, Comparison of Toxicity of Silver Nanoparticles in Ctenopharyngodon idella, Barbus grypus's (Astronorus ocellatus) and Cichlosoma severums, Journal of Marine Biology, Islamic Azad University, Ahvaz Branch, Year 2, Number 7 Pp. 45-51.


15. Alishahi, M. And Mesbah, M., Ghorbanpour, M., 2011. Comparison of nano-silver toxicity in four species of fish. Iranian Journal of Veterinary Medicine. Seventh period. No. 1. Page 41_36.


16. Bar-Ilan, O., Albrecht, R. M., Fako, V. and Furgeson, D. Y., 2009. Toxicity assessments of multisized gold and silver nanoparticles in zebrafish. Embryos Small, 5, 16,1897-1910.


17. Vajargah MF, Hossaini SA, Niazie EH, Hedayati A, Vesaghi MJ. Acute toxicity of two pesticides Diazinon and Deltamethrin on Tench (Tinca tinca) larvae and fingerling. International Journal of Aquatic Biology. 2013 May 29; 1(3):138-42.


18. Forouhar Vajargah M, Mohammadi Yalsuyi A, Hedayati A. Acute toxicity of povidone-iodine (Betadine) in common carp (Cyprinus carpio 1758). Pollution. 2017 Oct 1; 3(4): 589-93.