Optimizing exotic effective oil-hydrolyzing bacteria survival in different carriers
Subject Areas : Environmental pollutions (water, soil and air)
Mehran Salimi Bajestani
1
,
Soheila Ebrahimi
2
*
,
Reza Ghorbani Nasr Abadi
3
1 - Ph.D. Candidate, Dept. of Soil Science and Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2 - Associate Prof, Dept. of Soil Science and Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. *(Corresponding Author)
3 - Associate Prof., Dept. of Soil Science and Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Keywords: Bacteria carrier, peat, bran, biochar, Pseudomonas aeruginosa.,
Abstract :
Background and Objective: This research was conducted to investigate introduction feasibility of a carrier for exotic effective oil-hydrolyzing bacteria and optimizing suitable moisture and temperature factors proper for the mentioned carrier to preserve the bacteria.
Material and Methodology: Hence, three carriers of peat, bran and biochar were selected, bacteria number was monitored during a 6 month period after inoculation of exotic effective bacteria. Furthermore, two moisture levels of carrier moisture and temperature on bacteria persistence, two moisture levels of field capacity and 70% field capacity and two temperatures of 4 and 25 °C were applied and investigated on persistence of Pseudomonas aeruginosa.
Findings: All three carriers showed an increase in bacteria count at 25°C and field capacity during 2 weeks (bran and biochar) and one month (peat). However, monitoring effective depredating bacteria population during 6 month illustrated that peat, bran and biochar had the highest survival during 180 days at field capacity, which were 8.402, 8.205 and 8.116 (logCFU / g).
Discussion and Conclusion: Global standard for proper carrier, is the presence of bacteria population of 107 to 109 logCFU/g for 180 days. However, optimum temperature was first obtained 25°C at the beginning of the period, which was later changed to 4°C at the end. The lower temperature is optimum as a result of less toxic inhibitor gasses, reduction of bacteria activity and preservation of carrier nutrients during 180 day period. Optimum moisture condition at field capacity led to proper ventilation for growth and propagation of the bacteria.
On the other hand, noting the studied factors, peat, bran and biochar carriers had the highest bacteria population respectively. The main reason for population decrease may be attributed to heterotroph state of Pseudomonas aeruginosa and deficiency of organic matter in biochar and bran compared to peat.
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