Study on genetic variation of Therioaphis trifolii using RAPD-PCR method

Subject Areas : entomology and othea arthropods

M. Takalloozadeh ¹ , H. M. Takalloozadeh ² , A. Hosseini Pour ³ , A. M. Sarafrazi ⁴

1 - Department Of Biotechnology, College of Agriculture, Shahid Bahonar University of Kerman, Iran
2 - Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Iran
3 - Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Iran
4 - Insects Taxonomy Research Department, Iranian Research Institute of Plant Protection, Tehran, Iran

Received: 2011-06-21 Accepted : 2012-05-19 Published : 2013-05-22

Keywords:

Abstract :

Alfalfa (Medicago Sativa) is one of the most important forage plants in Iran. Several pests like aphids cause damage to these plants. One of the important aphids is the spotted alfalfa aphid (Therioaphis trifolii). In order to study the genetic variation of this pest, samples were taken from alfalfa fields of different provinces of Iran (Kerman, Fars, Kohkiloieyeh, Lorestan, Hamadan, Kermanshah, Kurdistan, West Azerbaijan, Markazi and Esfahan provinces) during 2007-2009. Seven RAPD primers were used in PCR test. At the range of 200-400 bp., 68 bands out of 88 had polymorphism. The primers A11 and CO4 had the highest polymorphisms that were 77.78 and 73.68 percent respectively. The related dendrogram showed 3 clusters and the second cluster had two sub clusters. In this research genetic differences were seen between collected samples from various regions, but no relationship was observed between these differences and altitudes, longitude, and position of the sampling locations.