َMicrogametophyte development in Leucantemum vulgare L.

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

zahra baghaeifar ¹ (گروه زیست شناسی دانشگاه پیام نور همدان)

Received: 2017-01-20 Accepted : 2017-03-13 Published : 2017-02-19

Keywords: development, Asteraceae, Pollen grain, Leucantemum vulgare, tapetum,

Abstract :

In this research, developmental stages of microsporogenesis and pollen grains development of leucantemum vulgare for Asteraceae family were studied. The flowers and buds in different developmental stages were removed, fixed in FAA70%, stored in 70% ethanol, embedded in paraffin and then sliced at 7 µm by microtome. Staining was carried out with Eosin and contrasted with Hematoxylin. The prepared slides were studied using light microscope. The result indicated that the formation and development of stamen primordum sooner than development of the pistil is done. The anthers were tetra-sporangiate. Pollen tetrads were tetragonal and tetrahedral (tetrahedral is more than tetragonal). Cytokinesis was synchronous. The mature pollen grains are spherical. 

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