Stereological study and measure serum malondyaldhyd, urea and creatinine with the effect of Ginger extract on renal damage caused by bisphenol A in NMRI mice

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Received: 2015-11-23 Accepted : 2016-03-10 Published : 2016-05-21

Keywords: kidney, Bisphenol A, Ginger extract, Stereology,

Abstract :

Bisphenol A (BPA), an endocrine disrupting compound largely used in plastic and paper industry, can induce reactive oxygen species (ROS) by increasing oxidative stress in many organs including kidney [1,2].The aim of this study was to investigate the protective effect of Ginger extract (GE) on kidney tissue and some serum biochemical parameters against toxicity induced with BPA. Adult male NMRI mice with mean body weight 32±3 g were randomly divided into 4 groups (n=6): Control, BPA (240mg/kg/day), GE (500ml/kg/day) and BPA + GE. Oral treatment was performed till 34 days. At the end of treatments, Body and left kidney weight were recorded. After fixation the tissue, tissue sections were prepared and stained with Haiden Han Azan method and studied stereological to evaluate the volume of kidney, cortex and medulla, renal tubules, renal corpuscle and its components. Serum malondialdehyde (MDA), creatinine and urea levels were also measured. Data were analyzed with One Way ANOVA test.  P<0.05 was considered as statistically significant. A significant increase in weight of kidney, volume of kidney, cortex, renal tubules and their epithelium and lumen, glomerolus, taft, renal corpuscle and rare of MDA, creatinine and urea in serum was found in mice treated with BPA compared to the control group (p<0.05). In BPA + GE group, Ginger significantly decreased the mentioned parameters compared to the BPA group (p<0.05). These findings suggest that Ginger extract by reduction in serum MDA, creatinine and urea, and tissue damage, appears to have a protective role in the Bisphenol A -induced injury in renal tissue.

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