Improvement of cell culture media and taxane production in suspension culture of Corylus avellana by low-intensity ultrasound

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Received: 2014-07-27 Accepted : 2014-12-30 Published : 2015-09-11

Keywords: Corylus avellana, ultrasound, Baccatin III, 10-Deacetylbaccatin III, phenylalanine ammonialyase Taxol,

Abstract :

Low-intensity ultrasound is known as a physical elicitor with a variety of biological effects on living cells. However, little information is available on the use of ultrasound in order to enhance biological processes. The present study was undertaken to clarify the effects of Low-energy ultrasound on the cell growth and secondary metabolites biosynthesis in suspension-cultured Coryllus avellana cell. Taxol, an anticancer drug which is usually extracted from Taxus sp., has been isolated from Coryllus avellana cells as well. The cells were grown in a modified LS medium and were exposed to ultrasound at power density of 4 mW/ cm2 for 4 to 40 min. Sonication of the cells for 20 min remarkably increased the yields of three major taxanes, i.e., paclitaxel, 10-deacetyl baccatin, and baccatin III (up to 6.07, 2.76, and 1.7 mg/kg, respectively). Enhancement of phenylalanine ammonialyase was also observed in ultrasound-treated cells. The results showed that the applied ultrasound had no significant effect on cell growth but, interestingly resulted in the increase of taxol biosynthesis by the cells. According to the results elicitation of Coryllus avellana with ultrasound can be suggested as a successful strategy for increase of the production of desired secondary metabolites.

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