Study of cytotoxic effects of alcoholic extract of salvia officinalis onThe 4T1 cell line derived from mouse mammary tumors in BALB/ c.
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Keywords: Cytotoxicity, sage (salvia officinalis), Taxol, Sage aqueous extract,
Abstract :
The aim of This study investigated the effects of cytotoxic plants sage (Salvia officinalis) on 4T1 cell line derived from mammary tumors in BALB / c mice is discussed. 4T1 cell line was prepared from Pastur Institue of Iran, after transferring to the University of Arak, Fused cells were frozen in dry ice, Then were cultured in medium RPMI1640, FBS10%, Were then placed in a CO2 incubator. Dried leaves of Sage were extracted using digestion method with the help of methanol and filtrated by filter paper. One part of extract was kept away, and to separate the non polar compounds, the rest of the extract was washed with non polar solvents (hexane, dichloromethane, ethyl acetate). Then the solvents were removed by rotary evaporator and then the desirable dilutions of extracts as well as Taxol were prepared. After 24 hours treatment of the cells with the extract and taxol the cell viability was evaluated by Trypan blue dye exclusion and MTT colorimetric assay.Aqueous extract and Taxol showed significant cytotoxic effects on breast cells cancerous. according to this study Aqueous extract of sage (Salvia officinalis)had a dose dependent cytotoxic effect on cancerous cells which was comparable to Taxol cytotoxicity, Metastasis is the most powerful class of 4T1 cell and a good model for studies of breast cancer.
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