Study the regeneration and analyze the karyotype of the planarian Schmidtea mediterranea
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Keywords: regeneration, Schmidtea mediterranea, karyotype, Neoblast, Planaria,
Abstract :
Having an unlimited capacity to regenerate tissues lost to age and injury has been studied and analyzed. Planarians are attended for more than 250 due to their ability of complex regenerative achievements dependent on a population of self-renewing adult stem cells called neoblasts. The key attribute of neoblasts are their capacity for imprecise self-renewal, their pluriputency and the ability of their progeny to explain differentiation and polarity signals and correctly replace lost organelsand tissues after damage. In this study, we explored the regeneration in planarian species of Schmidtea mediterranea. Here, study and analysis all planarians which are cut into 3 fragments including head, trunk, and tail regions after 1 week of starvation, put in 1ml PAM in 6 well-plates during two weeks. As regards, there are epimorphosis and morpholaxis regeneration in 14 days of planarian regeneration, all tissues and organs will completely repair and regenerate after injured. In the next step, the karyotype protocol which has set up in this research has shown 8 pairs of chromosomes in this species. Planarians because of having pluripotent stem cells will regenerate lost tissues and cells and during regeneration. Given that process the differentiation and undifferentiated stem cells is a prerequisite for the future therapy.
[1] Aboobaker, A. A. and D. Kao (2012). "A lack of commitment for over 500 million years: conserved animal stem cell pluripotency." The EMBO journal31(12): 2747-2749.
[2] Agata, K. (2003). "Regeneration and gene regulation in planarians." Current opinion in genetics & development13(5): 492-496.
[3] Agata, K., T. Tanaka, C. Kobayashi, K. Kato and Y. Saitoh (2003). "Intercalary regeneration in planarians." Developmental dynamics226(2): 308-316.
[4] Alvarado, A. S. (2004). "Planarians." Current Biology14(18): R737-R738.
[5] Alvarado, A. S., P. A. Newmark, S. M. Robb and R. Juste (2002). "The Schmidtea mediterranea database as a molecular resource for studying platyhelminthes, stem cells and regeneration." Development129(24): 5659-5665.
[6] Alvarado, A. S. and P. A. Tsonis (2006). "Bridging the regeneration gap: genetic insights from diverse animal models." Nature Reviews Genetics7(11): 873-884.
[7] Baguñà, J. (2012). "The planarian neoblast: the rambling history of its origin and some current black boxes." International Journal of Developmental Biology56(1-2-3): 19-37.
[8] Baguñà, J., S. Carranza, M. Pala, C. Ribera, G. Giribet, M. A. Arnedo, M. Ribas and M. Riutort (1999). "From morphology and karyology to molecules. New methods for taxonomical identification of asexual populations of freshwater planarians. A tribute to Professor Mario Benazzi."
[9] Bayascas, J., E. Castillo, A. Munoz-Marmol and E. Salo (1997). "Planarian Hox genes: novel patterns of expression during regeneration." Development124(1): 141-148.
[10] Benazzi, M. and G. Benazzi-Lentati (1976). "Animal Cytogenetics, vol. 1." Platyhelminthes. Gebr. Borntraeger, Berlin and Stuttgart.
[11] Cantarel, B. L., I. Korf, S. M. Robb, G. Parra, E. Ross, B. Moore, C. Holt, A. S. Alvarado and M. Yandell (2008). "MAKER: an easy-to-use annotation pipeline designed for emerging model organism genomes." Genome research18(1): 188-196.
[12] Carranza, S., J. Baguna and M. Riutort (1997). "Are the Platyhelminthes a monophyletic primitive group? An assessment using 18S rDNA sequences." Molecular Biology and Evolution14(5): 485-497.
[13] Cebrià, F., T. Adell and E. Saló (2010). "Regenerative medicine: lessons from planarians." Stem cell, regenerative medicine and cancer. Nova Science Publisher, Hauppauge, NY: 29-68.
[14] Forsthoefel, D. J. and P. A. Newmark (2009). "Emerging patterns in planarian regeneration." Current opinion in genetics & development19(4): 412-420.
[15] Forsthoefel, D. J., A. E. Park and P. A. Newmark (2011). "Stem cell-based growth, regeneration, and remodeling of the planarian intestine." Developmental biology356(2): 445-459.
[16] Gentile, L., F. Cebrià and K. Bartscherer (2011). "The planarian flatworm: an in vivo model for stem cell biology and nervous system regeneration." Disease Models and Mechanisms4(1): 12-19.
[17] Handberg-Thorsager, M., E. Fernández and E. Salo (2008). "1. Abstract 2. Stem cells and regeneration 2.1. Stem cells 2.2. Regeneration across the animal kingdom 2.3. Making use of regeneration 3. Planaria, a real regenerating model system 3.1. Historical overview." Frontiers in Bioscience13: 6374-6394.
[18] Mineta, K., M. Nakazawa, F. Cebrià, K. Ikeo, K. Agata and T. Gojobori (2003). "Origin and evolutionary process of the CNS elucidated by comparative genomics analysis of planarian ESTs." Proceedings of the National Academy of Sciences100(13): 7666-7671.
[19] Newmark, P. A. and A. S. Alvarado (2001). Regeneration in planaria, Wiley Online Library.
[20] Newmark, P. A. and A. S. Alvarado (2002). "Not your father's planarian: a classic model enters the era of functional genomics." Nature Reviews Genetics3(3): 210-219.
[21] Önal, P., D. Grün, C. Adamidi, A. Rybak, J. Solana, G. Mastrobuoni, Y. Wang, H. P. Rahn, W. Chen and S. Kempa (2012). "Gene expression of pluripotency determinants is conserved between mammalian and planarian stem cells." The EMBO journal31(12): 2755-2769.
[22] Oviedo, N. J., P. A. Newmark and A. Sánchez Alvarado (2003). "Allometric scaling and proportion regulation in the freshwater planarian Schmidtea mediterranea." Developmental dynamics226(2): 326-333.
[23] PRATS, J. (1991). "Analisi del contingut de DNA a planaria d'aigües dolces per citometria de flux i citoespectrofotmetria." Universitat de Barcelona.
[24] Randolph, H. (1892). "The regeneration of the tail in Lumbriculus." Journal of Morphology7(3): 317-344.
[25] Reddien, P. W. and A. S. Alvarado (2004). "Fundamentals of planarian regeneration." Annu. Rev. Cell Dev. Biol.20: 725-757.
[26] Reisinger, E. and S. Kelbetz (1964). "[FINE STRUCTURE AND DISCHARGE MECHANISM OF RHABDITES.]." Zeitschrift fur wissenschaftliche Mikroskopie und mikroskopische Technik65: 472-508.
[27] Saló, E. (2006). "The power of regeneration and the stem‐cell kingdom: freshwater planarians (Platyhelminthes)." Bioessays28(5): 546-559.
[28] Saló, E. and J. Baguna (2002). "Regeneration in planarians and other worms: new findings, new tools, and new perspectives." Journal of Experimental Zoology292(6): 528-539.
[29] Sluys, R., M. Kawakatsu, M. Riutort and J. Baguna (2009). "A new higher classification of planarian flatworms (Platyhelminthes, Tricladida)." Journal of Natural History43(29-30): 1763-1777.
[30] Takeda, H., K. Nishimura and K. Agata (2009). "Planarians maintain a constant ratio of different cell types during changes in body size by using the stem cell system." Zoological science26(12): 805-813.