Investigating the level of IP-10 gene expression in the peripheral blood of patients with severe type of corona virus (Covid-19) compared to the mild and recovered type of the disease
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Sanaz Paikar
1
(depaetment)
naghmeh Bahrami
2
(Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran)
robab rafiee tabatabaii
3
(department)
Abdolreza Mohamadnia
4
(Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran)
Keywords: Covid-19, biomarker, IP-10, gene expression,
Abstract :
SARS-CoV-2 is an enveloped, symmetrical virus with ridges on its membrane that give it a crown shape. The virus has a positive single-stranded RNA genome. The World Health Organization declared the coronavirus disease 2019 (COVID-19) a pandemic on March 11, 2020, due to the widespread development of the disease. In this research, the expression of IP-10 gene in the peripheral blood of patients with severe type of corona was evaluated in comparison with the mild and improved type of the disease.Peripheral blood was collected from 30 patients with severe infection, 30 patients with mild infection, and 30 recovered from the disease in the age group of 30-70 years. Real-time PCR was performed to check the expression of IP-10 gene in the samples. In this study, it was shown that the expression of the IP-10 gene decreased as the severity of the corona disease decreased, and therefore, this test can be used in the diagnosis and even follow-up of the disease.The studied gene is suitable for predicting the severity and prognosis of the disease, but to obtain better results, more samples should be examined.