Evaluation of miR-192 expression in lymph nodes of sarcoidosis patients by Real-time PCR method and comparison of their expression with the control group

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Samaneh sadat Rasi Varaei ¹ , Abdolreza Mohamadnia ² , Monireh Movahedi ³ , naghmeh Bahrami ⁴ , Sadegh Shirian ⁵

1 - Department of biochemistry, faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Chronic Respiratory Diseases Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 - Department of biochemistry, faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
4 - Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
5 - Department of pathology, school of veterinary medicine, shahrekord University, shahrekord, Iran

Received: 2023-12-31 Accepted : 2024-01-16 Published : 2024-05-05

Keywords: Tissues, Realtime PCR, lymph nodes, sarcoidosis,

Abstract :

Sarcoidosis is a disease with an unknown cause and the onset of the disease is often gradual. The disease may be asymptomatic or chronic. Examining the presence of factors such as micro RNAs may lead to better diagnoses of sarcoidosis and help to better understand the mechanism of sarcoidosis.This is a case-witness study that after obtaining permission from the ethics committee, 40 tissue samples from the lymph nodes of patients with sarcoidosis and 40 control samples were collected with the opinion of an expert doctor, and then RNA was extracted from the lymph nodes. Realtime PCR was done for miR-192 marker.miR-192 biomarker was positive in the lymph node samples of sarcoidosis patients in 28 out of 40 people. The positive rate of this biomarker in healthy people was 17 out of 40 people. miR-192 biomarker showed increased expression in sarcoidosis patients compared to healthy samples.According to the results of this study, miR-192 may be used as a molecular marker for sarcoidosis. It also has significant potential for diagnosis, prognosis and even treatment of sarcoidosis.