Effects of Chaste tree fruit extract on ovarian tissue and sex hormones of POF model mice

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

kiana ghorbani ¹ , Seyedeh Fatemeh Siadat ² , Rohollah Fathi ³ , Fatemeh Abedi Ghehi ⁴

1 - ISLAMIC AZAD UNIVERSITY SCIENCE AND RESEARCH BRACH Faculty of Science- Department of Biology
2 - Department of Biology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Embryology Department, Reproductive Biomedicine Research Center, Institute for Biology & Reproductive Medicine, Royan Institute,
4 - Embryology Department, Reproductive Biomedicine Research Center, Institute for Biology & Reproductive Medicine, Royan Institute,

Received: 2023-06-09 Accepted : 2023-08-05 Published : 2024-05-20

Keywords: Herbal medicines, Cyclophosphamide, Premature ovarian failure,

Abstract :

Premature ovarian failure (POF) is known as a heterogeneous disorder that leads to amenorrhea and ovarian failure before the age of 40 and causes infertility in young women. Herbal Medicines is traditionally used to treat hormonal problems in women. This study was conducted to investigate the effect of Chaste tree fruit hydro alcoholic extract on ovarian function and body weight of mice after POF induction in mice. In this study, 50 mice were divided into five groups (n=10) including the vitex group, POF and three experimental groups (creating POF model and gavage of vitex extract). After the effect of the extract, the ovarian tissue was studied with hematoxylin-eosin staining, and follicular count and serum level were checked. The results showed that the number of follicles, corpus luteum, and body weight of mice in the POF group were significantly reduced. Also, the serum level of FSH hormones increased in the POF group and the E2 hormone decreased significantly compared to the control group (p<0.05), which indicates the correct creation of POF models. After gavage of extract in POF mouse models, the serum level of hormones was adjusted and the number of ovarian follicles increased. The results of the study showed that vitex extract in the highest tested dose has positive effects on the number of follicles, body weight and sex hormones of mice in premature ovarian failure syndrome.