Comparative investigation of cytotoxicity effects of iron oxide, zinc oxide and silver nanoparticles on white blood cells and leukemic cell line HPB-ALL in Vitro

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Zahra Farahani ¹ , Kazem Parivar ² , nasim HAYATI ³ , Mona Farhadi ⁴

1 - Department of Biology, Faculty of Convergent Sciences and Technologies, Tehran Science and Research Unit, Islamic Azad University, Tehran
2 - Department of Biology, Faculty of Convergent Sciences and Technologies, Tehran Science and Research Unit, Islamic Azad University, Tehran
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

Received: 2022-12-17 Accepted : 2023-02-21 Published : 2024-02-12

Keywords: leukemia, white blood cells, , silver-zinc - Iron nanoparticles, apoptosis,

Abstract :

Treatment with nanoparticles has attracted the attention of many researchers. Due to their small size and easy absorption, nanoparticles can be a suitable option for treatment. In this study, a comparative study of the anticancer effect of silver nanoparticles, zinc oxide and iron oxide on leukemic cell line and white blood cells of HPB-ALL was done.Human white blood cells and leukemia cell line HPB-ALL were passaged. Iron oxide, zinc oxide and silver nanoparticles with different concentrations were added to the microplates containing the cells and the cells were treated with nanoparticles for 24 hours. PI was evaluated. The results obtained for leukemic cell line HPB-ALL and white blood cells were analyzed with the effect of three nanoparticles of silver, zinc oxide and iron oxide at a significant level (P<0.05). All three nanoparticles showed cytotoxic effects in both cell lines. The cytotoxicity effects of nanoparticles were higher in leukemic cell line HPB-ALL than in white blood cells. Among these nanoparticles, silver nanoparticles had a significant cytotoxic effect in HPB-ALL leukemic cell line compared to the other two nanoparticles. Evaluation of IC50, DNA damage and apoptosis induction in HPB-All leukemic cell line by silver nanoparticles was significantly higher than other two nanoparticles. According to the above results and further investigations of the penetration mechanism of each of the nanoparticles, it can be said that silver nanoparticles have the potential for therapeutic applications.